Results 71 to 80 of about 739,130 (263)
Template plasmids optimized for deletion of multiple genes in yeast Saccharomyces cerevisiae
Microbiology SpectrumFor Saccharomyces cerevisiae, gene knockout is routinely performed by transformation with a linear DNA cassette consisting of a selection marker gene flanked by upstream and downstream sequences homologous to a target gene.
Yi-He Feng +3 more
doaj +1 more source
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
Coordination Knockout Experiments
, 2014 Coordination Knockout ...
Heather J. Goldsby (563266) +3 more
core +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Knockout efficiency analysis of the FUT3 gene.
, 2022 (A) PCR results of knockout vector transfected cells, where Blank represents blank cells, and KO-1~4 are cells transfected with different knockout vectors. (B) Digestion results of PCR products of knockout vector transfected cells.
Song Gao (131209) +6 more
core +1 more source
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
, 2019 (A) Schematic of the dZDHHC8 genomic locus, indicating transcript isoforms and knockout regions. Two independent dZDHHC8 knockout lines were generated: knockout 1 (KO1) which removes the majority of dZDHHC8 as well as CG34450, and knockout 2 (KO2) which ...
Evangeline Kang (6316253) +2 more
core +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
The journey from gene knockout to clinical medicine: telotristat and sotagliflozin
, 2019 Marc S Rendell1,2 1Association of Diabetes Investigators, Omaha, NE 68131, USA; 2Rose Salter Medical Research Foundation, Newport Coast, CA 92657, USA Abstract: Gene knockout has been a powerful technique to evaluate the physiologic role of selected ...
Rendell MS
core