Results 11 to 20 of about 1,863,148 (392)

The eIF2A knockout mouse [PDF]

Cell Cycle, 2016
Eukaryotic initiation factor 2A (eIF2A) is a 65-kDa protein that was first identified in the early 1970s as a factor capable of stimulating initiator methionyl-tRNAi (Met-tRNAMeti) binding to 40S ribosomal subunits in vitro. However, in contrast to the eIF2, which stimulates Met-tRNAMeti binding to 40S ribosomal subunits in a GTP-dependent manner ...
A. Golovko, Artyom Kojukhov, Bo-Jhih Guan, B. Morpurgo, W. Merrick, B. Mazumder, M. Hatzoglou, A. Komar   +7 more
semanticscholar   +3 more sources

The Knockout Mouse Project [PDF]

Nature Genetics, 2004
Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes.
Austin, C., Battey, J., Bradley, A., Bucan, M., Capecchi, M., Collins, F., Dove, W., Duyk, G., Dymecki, S., Eppig, J., Grieder, F., Heintz, N., Hicks, G., Insel, T., Joyner, A., Koller, B., Lloyd, K., Magnuson, T., Moore, M., Nagy, A., Pollock, J., Roses, A., Sands, A., Seed, B., Skarnes, W., Snoddy, J., Soriano, P., Stewart, D., Stewart, F., Stillman, B., Varmus, H., Varticovski, L., Verma, I., Vogt, T., von Melchner, H., Witkowski, J., Woychik, R., Wurst, W., Yancopoulos, G., Young, S., Zambrowicz, B.   +40 more
openaire   +5 more sources

SIRT7 Deficiency Protects against Aging-Associated Glucose Intolerance and Extends Lifespan in Male Mice

Cells, 2022
Sirtuins (SIRT1–7 in mammals) are evolutionarily conserved nicotinamide adenine dinucleotide-dependent lysine deacetylases/deacylases that regulate fundamental biological processes including aging.
Tomoya Mizumoto, Tatsuya Yoshizawa, Yoshifumi Sato, Takaaki Ito, Tomonori Tsuyama, Akiko Satoh, Satoshi Araki, Kenichi Tsujita, Masaru Tamura, Yuichi Oike, Kazuya Yamagata   +10 more
doaj   +1 more source

A conditional counterselectable Piga knockout in mouse embryonic stem cells for advanced genome writing applications

iScience, 2022
Summary: Overwriting counterselectable markers is an efficient strategy for removing wild-type DNA or replacing it with payload DNA of interest. Currently, one bottleneck of efficient genome engineering in mammals is the shortage of counterselectable ...
Weimin Zhang, Ran Brosh, Laura H. McCulloch, Yinan Zhu, Hannah Ashe, Gwen Ellis, Brendan R. Camellato, Sang Yong Kim, Matthew T. Maurano, Jef D. Boeke   +9 more
doaj   +1 more source

A tecpr2 knockout mouse exhibits age-dependent neuroaxonal dystrophy associated with autophagosome accumulation

Autophagy, 2020
Mutations in the coding sequence of human TECPR2 were recently linked to spastic paraplegia type 49 (SPG49), a hereditary neurodegenerative disorder involving intellectual disability, autonomic-sensory neuropathy, chronic respiratory disease and ...
Bat-Chen Tamim-Yecheskel, Milana Fraiberg, Kamilya Kokabi, Saskia Freud, Oren Shatz, Letizia Marvaldi, Nemanja Subic, O. Brenner, M. Tsoory, R. Eilam-Altstadter, I. Biton, A. Savidor, N. Dezorella, G. Heimer, C. Behrends, B. Ben-Zeev, Z. Elazar   +16 more
semanticscholar   +1 more source

PTGES/PGE2 signaling links immunosuppression and lung metastasis in Gprc5a-knockout mouse model

Oncogene, 2020
Chronic inflammation has been linked to promotion of tumorigenesis and metastasis in lung. However, due to lack of a relevant animal model for characterization, the underlying mechanism remains elusive.
Tong Wang, Bo Jing, Dongliang Xu, Yueling Liao, Hongyong Song, Beibei Sun, Wenzheng Guo, Jianhua Xu, Kaimi Li, Min Hu, Shuli Liu, Jing Ling, Yanbin Kuang, Tuo Zhang, Siwei Zhang, F. Yao, Binhua P. Zhou, Jiong Deng   +17 more
semanticscholar   +1 more source

Knockout or Knock-in? A Truncated D2 Receptor Protein Is Expressed in the Brain of Functional D2 Receptor Knockout Mice

NeuroSci, 2021
Null mice for the dopamine D2 receptor (D2R) have been instrumental in understanding the function of this protein. For our research, we obtained the functional D2R knockout mouse strain described initially in 1997.
Natalia Sánchez, Montserrat Olivares-Costa, Marcela P González, Roberto Munita, Angélica P Escobar, Rodrigo Meza, Mauricio Herrera-Rojas, Jessica Albornoz, Gianluca Merello, María E Andrés   +9 more
doaj   +1 more source

CRISPR-mediated rapid generation of neural cell-specific knockout mice facilitates research in neurophysiology and pathology

Molecular Therapy: Methods & Clinical Development, 2021
Inducible conditional knockout mice are important tools for studying gene function and disease therapy, but their generation is costly and time-consuming.
Dan Xiao, Weifeng Zhang, Qing Wang, Xing Li, Yuan Zhang, Javad Rasouli, Giacomo Casella, Bogoljub Ciric, Mark Curtis, Abdolmohamad Rostami, Guang-Xian Zhang   +10 more
doaj   +1 more source

Role of ZBED3 in cortex cerebral development through conventional Zbed3 knockout mouse [PDF]

Jichu yixue yu linchuang, 2020
Objective To explore the role of zinc finger BED domain-containing protein 3, a member of zinc finger domain protein superfamily, in cortex cerebral development through conventional Zbed3 knockout mouse.
SUN Chang-jie, SHU Peng-cheng, PENG Xiao-zhong
doaj  

Knockout of reactive astrocyte activating factors slows disease progression in an ALS mouse model

Nature Communications, 2020
Reactive astrocytes have been implicated in the pathogenesis of neurodegenerative diseases, including a non-cell autonomous effect on motor neuron survival in ALS.
Kevin A. Guttenplan, Maya K. Weigel, Drew I. Adler, Julien Couthouis, SA Liddelow, A. Gitler, B. Barres   +6 more
semanticscholar   +1 more source