Results 21 to 30 of about 415,720 (292)
iScience, 2022 Summary: Overwriting counterselectable markers is an efficient strategy for removing wild-type DNA or replacing it with payload DNA of interest. Currently, one bottleneck of efficient genome engineering in mammals is the shortage of counterselectable ...
Weimin Zhang +9 more
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Generation of Human CRY1 and CRY2 Knockout Cells Using Duplex CRISPR/Cas9 Technology
Frontiers in Physiology, 2019 Circadian clocks are endogenous oscillators essential for orchestrating daily rhythms in physiology, metabolism and behavior. While mouse models have been instrumental to elucidate the molecular mechanism of circadian rhythm generation, our knowledge ...
Teresa Börding +10 more
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CRISPR/Cas9-mediated generation of a Plac8 knockout mouse model
Laboratory Animal Research, 2018 Placenta specific 8 (PLAC8, also known as ONZIN) is a multi-functional protein that is highly expressed in the intestine, lung, spleen, and innate immune cells, and is involved in various diseases, including cancers, obesity, and innate immune deficiency.
HyunJeong Lee +6 more
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Mouse genetics: Catalogue and scissors [PDF]
BMB Reports, 2012 Phenotypic analysis of gene-specific knockout (KO) mice hasrevolutionized our understanding of in vivo gene functions. Asthe use of mouse embryonic stem (ES) cells is inevitable forconventional gene targeting, the generation of knockout miceremains a ...
Han-Woong Lee
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Analysis of genome-wide knockout mouse database identifies candidate ciliopathy genes
Scientific Reports, 2022 We searched a database of single-gene knockout (KO) mice produced by the International Mouse Phenotyping Consortium (IMPC) to identify candidate ciliopathy genes.
Kendall Higgins +35 more
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Frontiers in Endocrinology, 2011 Brain aromatase is expressed only in the nerve cells of specific brain regions with a transient peak at the neonatal period when sexual behaviors become organized. Aromatase knockout (ArKO) mouse, generated to give light on the physiological functions of
Nobuhiro eHarada +2 more
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Production of knockout mouse lines with Cas9
Methods, 2021 Knockout mice are used extensively to explore the phenotypic effects of mammalian gene dysfunction. With the application of RNA-guided Cas9 nuclease technology for the production of knockout mouse lines, the time, as well as the resources needed, to progress from identification of a gene of interest to production of a knockout line is significantly ...
Marina Gertsenstein, Lauryl M. J. Nutter
openaire +3 more sources
Absence of BBSome function leads to astrocyte reactivity in the brain
Molecular Brain, 2019 In humans, dysfunctional primary cilia result in Bardet-Biedl syndrome (BBS), which presents with clinical features including intellectual disabilities, obesity, and retinal degeneration, and, in mouse models, the added feature of hydrocephalus.
Minati Singh +3 more
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Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia
FEBS Letters, EarlyView.This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley +1 more source
Journal of Pharmacological Sciences, 2017 Glutamate excitotoxicity mediated by N-methyl-d-aspartate (NMDA) receptors is an important cause of retinal ganglion cell death in glaucoma. To elucidate whether apelin protects against retinal neuronal cell death, we examined protective effects of ...
Yuki Ishimaru +6 more
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