Results 71 to 80 of about 103,281 (241)

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

open access: yesFEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Optimization study of raman effect detection versus fluorescence: application to art [PDF]

open access: yes, 2011
English: Nowadays, Raman spectroscopy has become a very important tool for identifying chemical compounds and their molecular structures since it is non-destructive, has null or near null requirements of sample preparation, and gives results very fast ...
Martí Edo, Albert
core   +2 more sources

Películas de ZnO para sensado de gas CO2 [PDF]

open access: yes, 2020
In present work is reported the zinc oxide (ZnO) deposit on corning glass substrates at 400°C by sol-gel spin coating technique. The optical effect of ZnO films was analyzed by transmittance measurements taken with help of UV-vis spectrophotometer.
Herandez Zanabria, Angélica Guadalupe   +3 more
core   +2 more sources

Dapagliflozin prevents methylglyoxal‐induced retinal cell death in ARPE‐19 cells

open access: yesFEBS Open Bio, EarlyView.
Diabetic macular oedema is a diabetes complication of the eye, which may lead to permanent blindness. ARPE‐19 are human retinal cells used to study retinal diseases and potential therapeutics. Methylglyoxal is a compound increased in uncontrolled diabetes due to elevated blood glucose.
Naina Trivedi   +7 more
wiley   +1 more source

Levantamiento arquitectónico y estudio físico-constructivo del "Molí Paperer" [PDF]

open access: yes, 2010
El molino papelero de Martorell data del 1739. Desde su origen hasta la actualidad, ha sufrido una serie de cambios provocados tanto por las necesidades industriales de cada momento, como por las constantes riadas que se sucedían en Martorell.
Pereira Sánchez, Ana   +1 more
core  

Validación de máquina briqueteadora para el aprovechamiento de la cascarilla de café como combustible [PDF]

open access: yes, 2013
La investigación contó con el apoyo técnico y financiero de la Fundación para el Desarrollo Tecnológico Agropecuario y Forestal de Nicaragua (FUNICA) y su finalidad fue validar una máquina briqueteadora de tornillo helicoidal para el aprovechamiento de ...
Amador, Aura   +3 more
core   +4 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Análisis Geométrico y Microestructural de Recubrimientos de NiCoCrAlYTa proyectados por láser sobre láminas de acero inoxidable AISI 316L

open access: yes, 2014
[EN] In this work, the NiCoCrAlYTa Alloy was deposited by laser cladding with different processing parameters (flow rate of powder supplied, feed speed and laser power), in order to analyze the effects of processing on the geometry, microstructure and properties of the coating.
Pereira F, Juan C   +3 more
openaire   +3 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

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