Results 111 to 120 of about 6,064,561 (323)

On-demand delivery of single DNA molecules using nanopipettes [PDF]

, 2015
Understanding the behavioral properties of single molecules or larger scale populations interacting with single molecules is currently a hotly pursued topic in nanotechnology.
Actis, P, Edel, JB, Ivanov, AP, Jönsson, P, Klenerman, DK, Korchev, Y   +5 more
core   +1 more source

Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering

Proceedings of the National Academy of Sciences of the United States of America, 2019
Significance Imaging flow cytometry is a powerful tool for analyzing every single cell in a large heterogeneous population but relies on fluorescent labeling, which comes with cytotoxicity, nonspecific binding, and interference with natural cellular ...
Yuta Suzuki, K. Kobayashi, Y. Wakisaka, D. Deng, Shunji Tanaka, Chun-Jung Huang, Cheng Lei, Chia‐Wei Sun, Hanqin Liu, Yasuhiro Fujiwaki, Sangwook Lee, A. Isozaki, Y. Kasai, T. Hayakawa, S. Sakuma, F. Arai, Kenichi Koizumi, H. Tezuka, M. Inaba, K. Hiraki, Takuro Ito, Misa Hase, S. Matsusaka, K. Shiba, Kanako Suga, Masako Nishikawa, M. Jona, Y. Yatomi, Yaxiaer Yalikun, Yo Tanaka, Takeaki Sugimura, Nao Nitta, K. Goda, Y. Ozeki   +33 more
semanticscholar   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Multiphoton Label-Free ex-vivo imaging using a custom-built dual-wavelength microscope with chromatic aberrations compensation

, 2017
Label-Free Multiphoton Microscopy is a very powerful optical microscopy that can be applied to study samples with no need for exogenous fluorescent probes, keeping the main benefits of a Multiphoton approach, like longer penetration depths and intrinsic ...
Armani, Andrea, Borile, Giulia, Filippi, Andrea, Gerosa, Gino, Gintoli, Michele, Iop, Laura, Romanato, Filippo, Sandri, Marco, Sasso, Eleonora Dal   +8 more
core   +1 more source

Label-free segmentation of co-cultured cells on a nanotopographical gradient [PDF]

, 2013
The function and fate of cells is influenced by many different factors, one of which is surface topography of the support culture substrate. Systematic studies of nanotopography and cell response have typically been limited to single cell types and a ...
Alberts B., Anderson D. G., Anderson D. G., Bakker R. M., Boss J., Cardona A., Cho B. H., Csaderova L., Curtis A. S. G., Dalby M. J., Dalby M. J., De Mel A., Ding Y., Gadegaard N., Gadegaard N., Gallant N. D., Geysen H. M., Glassford S., John Stormonth-Darling, Jones T. R., Jones T. R., Kamentsky L., Khetani S. R., Kirkpatrick C. J., Kolind K., Kunzler T. P., Mathis O. Riehle, Matthew J. Dalby, Ngo J. T., Nikolaj Gadegaard, Paul M. Reynolds, Pedersen R. H., Rasmus H. Pedersen, Reynolds P. M., Rivera Gil P., Schift H., Simon C. G., Stormonth-Darling J. M., Unadkat H. V, Vickaryous M. K., Yang J., Zelzer M.   +41 more
core   +1 more source

ANPELA: analysis and performance assessment of the label-free quantification workflow for metaproteomic studies

Briefings Bioinform., 2019
Label-free quantification (LFQ) with a specific and sequentially integrated workflow of acquisition technique, quantification tool and processing method has emerged as the popular technique employed in metaproteomic research to provide a comprehensive ...
Jing Tang, Jianbo Fu, Yunxia Wang, Bo Li, Yinghong Li, Qingxia Yang, Xuejiao Cui, Jiajun Hong, Xiaofeng Li, Yuzong Chen, Weiwei Xue, Feng Zhu   +11 more
semanticscholar   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source

Microfiber coupler based label-free immunosensor

Optics Express, 2014
Optical microfibers and related structures which incorporate large evanescent field and minimal size offer new opportunities for biosensing applications. In this paper we report the development of an immunosensor based on a tapered microfiber coupler embedded in a low refractive index polymer.
Bo, Lin, O'Mahony, Christy Charlton, Semenova, Yuliya, Gilmartin, Niamh, Wang, Pengfei, Farrell, Gerald   +5 more
openaire   +3 more sources