Results 91 to 100 of about 518,105 (292)

Label‐Free Microfluidic Apheresis of Circulating Tumor Cell Clusters

Advanced Science
Screening liters of blood (i.e., apheresis) represents a generalized approach to promote the reliable access to circulating tumor cell clusters (CTCCs), which are known to be highly metastasis‐competent, yet ultrarare.
Li Zhan, Jon Edd, Avanish Mishra, Mehmet Toner   +3 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Microfiber coupler based label-free immunosensor

Optics Express, 2014
Optical microfibers and related structures which incorporate large evanescent field and minimal size offer new opportunities for biosensing applications. In this paper we report the development of an immunosensor based on a tapered microfiber coupler embedded in a low refractive index polymer.
Bo, Lin, O'Mahony, Christy Charlton, Semenova, Yuliya, Gilmartin, Niamh, Wang, Pengfei, Farrell, Gerald   +5 more
openaire   +3 more sources

Highly sensitive and label-free digital detection of whole cell E. coli with interferometric reflectance imaging [PDF]

, 2019
Bacterial infectious diseases are a major threat to human health. Timely and sensitive pathogenic bacteria detection is crucial in identifying the bacterial contaminations and preventing the spread of infectious diseases.
Bhuiya, Abdul Muyeed, Dupuis, Julia R., Ekiz Kanik, Fulya, Geib, Matthew T., Gong, Emily S., Ozkumur, Ayca Yalcin, Zaraee, Negin, Ünlü, M. Selim, Ünlü, Nese Lortlar   +8 more
core   +2 more sources

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

NOVEL FREE LABEL BOTULINUM APTASENSOR BASED ON CAPACITANCE METHOD

Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry, 2016
A feasible and fast method to fabricate Botulinum neurotoxin serotype A (BoNT/A) biosensor was investigated by graphene-carbon paste nanocomposite electrode. In the present work, the graphene was synthesis by chemical method and characterized by SEM. The
JAMAL RASHIDIANI, SAYED JAEFAR MOOSAVI, KHADIJEH ESKANDARI, FIROOZ EBRAHIMI   +3 more
doaj  

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

, 2017
We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 {\mu}m axial and 0.4 {\mu}m lateral resolution maintained over a depth of 40 {\mu}m, while preserving the advantages of Fourier domain OCM.
Bouwens, Arno, Coquoz, Séverine, Descloux, Adrien, Extermann, Jérôme, Lasser, Theo, Marchand, Paul J., Nguyen, David, Sison, Miguel, Szlag, Daniel   +8 more
core   +1 more source

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source