Results 81 to 90 of about 518,105 (292)

Rare-cell enrichment by a rapid, label-free, ultrasonic isopycnic technique for medical diagnostics [PDF]

, 2014
One significant challenge in medical diagnostics lies in the development of label-free methods to separate different cells within complex biological samples.
Bell, Bourquin, Chappuis, Franke, Friend, Grover, Kumar, Leif, Lejon, Minion, Nash, Raghavan, Reboud, Rogers, Tan, Urdea, Wilson, Yager, Yatsushiro   +18 more
core   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

Fast label-free multilayered histology-like imaging of human breast cancer by photoacoustic microscopy [PDF]

, 2017
The goal of breast-conserving surgery is to completely remove all of the cancer. Currently, no intraoperative tools can microscopically analyze the entire lumpectomy specimen, which results in 20 to 60% of patients undergoing second surgeries to achieve ...
Aft, Rebecca L, Hai, Pengfei, Novack, Deborah V, Pleitez, Miguel A, Wang, Lihong V, Wong, Terence T.W., Zhang, Chi, Zhang, Ruiying   +7 more
core   +3 more sources

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Label‐Free Photonic Biosensors: Key Technologies for Precision Diagnostics

ChemistryEurope
With the rise of multidrug‐resistant pathogens, emerging zoonotic viruses, and the increasing prevalence of cancer, the modernization of clinical diagnostics has become a critical priority.
Maria Soler, Laura M. Lechuga
doaj   +1 more source

Label-free optical detection of single enzyme-reactant reactions and associated conformational changes

, 2017
Monitoring the kinetics and conformational dynamics of single enzymes is crucial in order to better understand their biological functions as these motions and structural dynamics are usually unsynchronized among the molecules.
Baaske, Martin D., Kim, Eugene, Schuldes, Isabel, Vollmer, Frank, Wilsch, Peter S.   +4 more
core   +1 more source

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Multiphoton Label-Free ex-vivo imaging using a custom-built dual-wavelength microscope with chromatic aberrations compensation

, 2017
Label-Free Multiphoton Microscopy is a very powerful optical microscopy that can be applied to study samples with no need for exogenous fluorescent probes, keeping the main benefits of a Multiphoton approach, like longer penetration depths and intrinsic ...
Armani, Andrea, Borile, Giulia, Filippi, Andrea, Gerosa, Gino, Gintoli, Michele, Iop, Laura, Romanato, Filippo, Sandri, Marco, Sasso, Eleonora Dal   +8 more
core   +1 more source

Label-free photoacoustic microscopy of cytochromes [PDF]

, 2013
Photoacoustic microscopy (PAM) has achieved submicron lateral resolution in showing subcellular structures; however, relatively few endogenous subcellular contrasts have so far been imaged. Given that the hemeprotein, mostly cytochromes in general cells,
Wang, Lihong V., Xia, Younan, Yao, Da-Kang, Zhang, Chi, Zhang, Yu Shrike   +4 more
core   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source