Results 101 to 110 of about 518,105 (292)
Simple quantitation and spatial characterization of label free cellular images
HeliyonLabel-free imaging is routinely used during cell culture because of its minimal interference with intracellular biology and capability of observing cells over time.
Vincent C.J. de Boer, Xiang Zhang
doaj +1 more source
Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor
Bioengineering, 2015 A thermoelectric biosensor for the detection of L-glutamate concentration was developed. The thermoelectric sensor is integrated into a micro-calorimeter which measures the heat produced by biochemical reactions.
Varun Lingaiah Kopparthy +2 more
doaj +1 more source
FEBS Letters, EarlyView.In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai +9 more
wiley +1 more source
Label-free and label-based electrochemical detection of disease biomarker proteins
ADMET and DMPKIntroduction: Biosensors, analytical devices integrating biological sensing elements with physicochemical transducers, have gained prominence as rapid and convenient tools for monitoring human health status using biochemical analytes.
Tias Febriana Hanifa Lestari +7 more
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Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Real-time, step-wise, electrical detection of protein molecules using dielectrophoretically aligned SWNT-film FET aptasensors [PDF]
Aptamer functionalized addressable SWNT-film arrays between cantilever electrodes were successfully developed for biosensor applications. Dielectrophoretically aligned SWNT suspended films made possible highly specific and rapid detection of target ...
An, T, HAHN, SEI KWANG, Kim, KS, Lim, G
core +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Nature Methods, 2009 Researchers develop a high-sensitivity, label-free imaging technology based on stimulated Raman scattering.
openaire +1 more source
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
FEBS Letters, EarlyView.This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel +13 more
wiley +1 more source