Results 121 to 130 of about 6,064,561 (323)

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate‐binding pocket

FEBS Letters, EarlyView.
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley   +1 more source

Label-free and label-based electrochemical detection of disease biomarker proteins

ADMET and DMPK
Introduction: Biosensors, analytical devices integrating biological sensing elements with physicochemical transducers, have gained prominence as rapid and convenient tools for monitoring human health status using biochemical analytes.
Tias Febriana Hanifa Lestari, Irkham Irkham, Uji Pratomo, Shabarni Gaffar, Salma Nur Zakiyyah, Isnaini Rahmawati, Seda Nur Topkaya, Yeni Wahyuni Hartati   +7 more
doaj   +1 more source

High-throughput label-free molecular fingerprinting flow cytometry

Science Advances, 2019
High-throughput Raman flow cytometry is developed to rapidly probe molecular fingerprints of numerous cells without labels. Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations ...
K. Hiramatsu, T. Ideguchi, Yusuke Yonamine, Sangwook Lee, Yizhi Luo, K. Hashimoto, Takuro Ito, Misa Hase, Jee-Woong Park, Y. Kasai, S. Sakuma, T. Hayakawa, F. Arai, Yuta Hoshino, K. Goda   +14 more
semanticscholar   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Simple quantitation and spatial characterization of label free cellular images

Heliyon
Label-free imaging is routinely used during cell culture because of its minimal interference with intracellular biology and capability of observing cells over time.
Vincent C.J. de Boer, Xiang Zhang
doaj   +1 more source

Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor

Bioengineering, 2015
A thermoelectric biosensor for the detection of L-glutamate concentration was developed. The thermoelectric sensor is integrated into a micro-calorimeter which measures the heat produced by biochemical reactions.
Varun Lingaiah Kopparthy, Siva Mahesh Tangutooru, Eric J. Guilbeau   +2 more
doaj   +1 more source

Mechanisms of parasite‐mediated disruption of brain vessels

FEBS Letters, EarlyView.
Parasites can affect the blood vessels of the brain, often causing serious neurological problems. This review explains how different parasites interact with and disrupt these vessels, what this means for brain health, and why these processes matter. Understanding these mechanisms may help us develop better ways to prevent or treat brain infections in ...
Leonor Loira, Sílvia Arroz‐Madeira, Cláudio A. Franco, Sara Silva Pereira   +3 more
wiley   +1 more source

NOVEL FREE LABEL BOTULINUM APTASENSOR BASED ON CAPACITANCE METHOD

Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry, 2016
A feasible and fast method to fabricate Botulinum neurotoxin serotype A (BoNT/A) biosensor was investigated by graphene-carbon paste nanocomposite electrode. In the present work, the graphene was synthesis by chemical method and characterized by SEM. The
JAMAL RASHIDIANI, SAYED JAEFAR MOOSAVI, KHADIJEH ESKANDARI, FIROOZ EBRAHIMI   +3 more
doaj  

Time after time – circadian clocks through the lens of oscillator theory

FEBS Letters, EarlyView.
Oscillator theory bridges physics and circadian biology. Damped oscillators require external drivers, while limit cycles emerge from delayed feedback and nonlinearities. Coupling enables tissue‐level coherence, and entrainment aligns internal clocks with environmental cues.
Marta del Olmo, Carolin Ector, Hanspeter Herzel   +2 more
wiley   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source