Results 131 to 140 of about 4,185,007 (300)

Label‐Free Molecular Characterization of Protein Aggregates in Differentiated Astrocytes

Advanced Science
Astrocyte cell differentiation to their characteristic starlike morphology with the expression of proteins in microdomains, critical for normal brain function, occurs naturally in‐vivo but can be affected in pathological condition or in cell culture in ...
Panagis D. Samolis, Chiara Lazzarini, Rahmetullah Durgun, Barbara Barile, Giorgia Conte, Tamara Posati, Marco Caprini, Grazia Paola Nicchia, Valentina Benfenati, Michelle Y. Sander   +9 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

From superior contrast to super resolution label free optical microscopy

npj Imaging
Label-free optical microscopy utilizes the information encoded in light scattered off unlabeled particles to generate the images. This review article starts off with a discussion on how this light matter interaction gives rise to the issues of poor ...
Nikhil Jayakumar, Balpreet Singh Ahluwalia   +1 more
doaj   +1 more source

Source data for "Instrument-agnostic optimization of innate fluorescence bands for label-free cell sorting"

Source data for "Instrument-agnostic optimization of innate fluorescence bands for label-free cell sorting"This work was funded by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
Yutaka Yawata, 10586457, 0000-0002-1061-5792   +2 more
core   +1 more source

Label-Free CRISPR/Cas9 Assay for Site-Specific Nucleic Acid Detection

, 2019
The development of the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has become a revolutionary step for genome engineering because it enables modification of target genomes.
Min Jiang, Rui Liu, Rui Liu (54031), Jianyu Hu, Min Jiang (56794), Jianyu Hu (2614810), Yi Lv, Yi Lv (344023)   +7 more
core   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Information‐Distilled Generative Label‐Free Morphological Profiling Encodes Cellular Heterogeneity

Advanced Science
Image‐based cytometry faces challenges due to technical variations arising from different experimental batches and conditions, such as differences in instrument configurations or image acquisition protocols, impeding genuine biological interpretation of ...
Michelle C. K. Lo, Dickson M. D. Siu, Kelvin C. M. Lee, Justin S. J. Wong, Maximus C. F. Yeung, Michael K. Y. Hsin, James C. M. Ho, Kevin K. Tsia   +7 more
doaj   +1 more source

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

FEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui, Agnes Banreti, Armelle L. Ernst, Guillaume Leseigneur, Uwe J. Meierhenrich   +4 more
wiley   +1 more source

Label-free 3D visualization and quantification of endogenous and exogenous intracellular particles in single cells by phase-contrast holographic flow tomography

, 2023
Detection and quantification of intracellular structures is fundamental in biomedical sciences. New emerging inspection tools based on holographic microscopy and quantitative phase imaging can give answers to such critical demands. Holographic tomography
Lisa Miccio, Luisa Iommarini, Ivana Kurelac, Francesco Merola, Giuseppe Cesare Lama, Simonetta Grilli, Rachele Castaldo, Martina Mugnano, Daniele Pirone, Daniele Sirico, Giannandrea Pasquinelli, Pietro Ferraro, Sabrina Valente, Pasquale Memmolo, Vittorio Bianco, Danila del Giudice, Silvia Lemma   +16 more
core  

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source