Results 121 to 130 of about 4,187,542 (302)
Label-free volumetric histopathology of thick-tissue slides - normal pancreatic duct
, 2020 Label-free volumetric histopathology of thick-tissue slides - normal pancreatic ...
yongkeun park (8695308)
core +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Label‐Free Molecular Characterization of Protein Aggregates in Differentiated Astrocytes
Advanced ScienceAstrocyte cell differentiation to their characteristic starlike morphology with the expression of proteins in microdomains, critical for normal brain function, occurs naturally in‐vivo but can be affected in pathological condition or in cell culture in ...
Panagis D. Samolis +9 more
doaj +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
Label-free 3D molecular imaging of living tissues using Raman spectral projection tomography
Nature CommunicationsThe ability to image tissues in three dimensions (3D) with label-free molecular contrast at the mesoscale would be a valuable capability in biology and biomedicine.
Elzbieta Stepula +10 more
doaj +1 more source
Source data for "Instrument-agnostic optimization of innate fluorescence bands for label-free cell sorting"This work was funded by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
Yutaka Yawata +2 more
core +1 more source
Label-Free CRISPR/Cas9 Assay for Site-Specific Nucleic Acid Detection
, 2019 The development of the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has become a revolutionary step for genome engineering because it enables modification of target genomes.
Min Jiang +7 more
core +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
From superior contrast to super resolution label free optical microscopy
npj ImagingLabel-free optical microscopy utilizes the information encoded in light scattered off unlabeled particles to generate the images. This review article starts off with a discussion on how this light matter interaction gives rise to the issues of poor ...
Nikhil Jayakumar +1 more
doaj +1 more source
Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers
FEBS Letters, EarlyView.Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui +4 more
wiley +1 more source