Results 201 to 210 of about 1,374,270 (261)

Improved inter-lab precision after CML international scale adoption: a 15-year history of BCR::ABL1 proficiency testing. [PDF]

Leukemia
Kim AS, Long T, Oldridge DA, Jennings LJ, Ding Y, Vasalos P, Watt C, Press RD.   +7 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Performance of carbapenemase screening algorithms for detection of OXA-244-producing <i>Escherichia coli</i>. [PDF]

JAC Antimicrob Resist
Keizer KN, Harryvan TJ, Maat I, Vainio SJ, Vlek ALM, Melles DC, Rentenaar RJ, Groothuis DE, Kluytmans JAJW, Frakking FNJ.   +9 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Aquaporin-4 Orchestrates Muscle Cell Differentiation and Satellite Cell Preservation: A Novel Target in Age-Related Muscle Decline. [PDF]

J Cachexia Sarcopenia Muscle
Girón MD, Manzano M, Pereira SL, Reinoso A, Burgio C, Montero-Martin N, Jiménez-Jiménez G, Argilés JM, Rueda R, López-Pedrosa JM, Salto R.   +10 more
europepmc   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Benchmarking RNA-seq with the Quartet and MAQC reference materials to establish best practices for accurate alternative splicing analysis. [PDF]

Nat Commun
Wang D, Zhao J, Chen Q, Han Y, Liu Y, Zhang Y, Liu C, Hou W, Yu Y, Shi L, Zheng Y, Li J, Zhang R.   +12 more
europepmc   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Diagnosis and monitoring of thrombotic thrombocytopenic purpura: Guidance for laboratories from the British Society for Haematology. [PDF]

Br J Haematol
MacDonald S, Pitchford T, Singh D, Fretwell R, Jenkins PV, Lawrence C, Gooding R, Platton S, BSH Committee.   +8 more
europepmc   +1 more source