Results 91 to 100 of about 128,431 (240)

Transient repression of the lac operon — the effect of a lac promoter deletion [PDF]

FEBS Letters, 1970
Experiments have been done to show whether the lac promoter delection L1, which partly alleviates catabolite repression, also affects transient repression of lac. In stain L1/F′M15 all of the β‐galactosidase is synthesized from a chromosomal gene cis to L1, whereas 98% of the thiogalactosidase transacetylase is synthesized from an episomal gene cis to ...
openaire   +3 more sources

Polycistronic Effects of Catabolite Repression on the lac Operon [PDF]

Journal of Bacteriology, 1972
The catabolite repression caused by glucose and glucose-6-phosphate has been studied for both β-galactosidase and thiogalactoside transacetylase, the products of the operator proximal and distal cistrons of the lac operon, respectively. We find that both cistrons are affected coordinately by this form of repression.
Allen E. Silverstone, Boris Magasanik
openaire   +3 more sources

Sigma Factors as Potential Targets to Enhance Recombinant Protein Expression

Biotechnology and Bioengineering, Volume 122, Issue 6, Page 1598-1607, June 2025.
ABSTRACT The transcriptional factors control the expression of many genes and represent an important layer of complexity in cell factories. However, the effect of individual sigma factor deletions from a biomanufacturing perspective has not been addressed. In this contribution, growth, green fluorescence protein (GFP) expression, and oxygen consumption
Laura Pohlen, Emily García, Luz María Martínez, Noemí Flores, Jochen Büchs, Guillermo Gosset, Alvaro R. Lara   +6 more
wiley   +1 more source

Quantitative Characterization of Combinatorial Transcriptional Control of the Lactose Operon of E. coli [PDF]

, 2007
It is the goal of systems biology to understand the behavior of the whole in terms of the knowledge of the parts. This is hard to achieve in many cases due to the difficulty of characterizing the many constituents and their complex web of interactions ...
Hwa, T., Kuhlman, T. E., Saier Jr., M. H., Zhang, Z.   +3 more
core   +2 more sources

Engineering Chromosome Bridges Through CRISPR/Cas9 to Decipher the Impact of Intercentromeric Distance on Resolution Dynamics

The FASEB Journal, Volume 39, Issue 9, 15 May 2025.
Chromosome bridge resolution during mitosis is influenced by the length of the bridging chromatin. Using CRISPR/Cas9, we engineered chromosome bridges with precisely defined intercentromeric distances. Results on the progression of these bridges during mitosis revealed that longer bridges require greater kinetochore separation to break than shorter ...
Teresa Anglada, Marina Rodriguez‐Muñoz, Núria Pulido‐Artola, Anna Genescà   +3 more
wiley   +1 more source

Interplay of gene expression noise and ultrasensitive dynamics affects bacterial operon organization.

PLoS Computational Biology, 2012
Bacterial chromosomes are organized into polycistronic cotranscribed operons, but the evolutionary pressures maintaining them are unclear. We hypothesized that operons alter gene expression noise characteristics, resulting in selection for or against ...
J Christian J Ray, Oleg A Igoshin
doaj   +1 more source

Analysis of the lactose metabolism in E. coli using sum-of-squares decomposition [PDF]

, 2005
We provide a system-theoretic analysis of the mathematical model of lactose induction in E.coli which predicts the level of lactose induction into the cell for specified values of external lactose.
Ahmadzadeh, Ali, Halasz, Adam, Jadbabaie, Ali, Kumar, Vijay, Prajna, Stephen   +4 more
core  

Parameter estimation for Boolean models of biological networks [PDF]

, 2009
Boolean networks have long been used as models of molecular networks and play an increasingly important role in systems biology. This paper describes a software package, Polynome, offered as a web service, that helps users construct Boolean network ...
Abdul S. Jarrah, Albert, Barrett, Brandilyn Stigler, Cantone, Chaves, Davidich, Eickmeyer, Elena Dimitrova, Faure, Franziska Hinkelmann, Gupta, Herrgard, Jarrah, Kauffman, Kauffman, Kauffman, Kauffman, Laubenbacher, Li, Li, Luis David García-Puente, Michael Stillman, Mora, Paola Vera-Licona, Reinhard Laubenbacher, Saez-Rodriguez, Samal, Stigler, Sturmfels, Thomas, Zhang   +31 more
core   +3 more sources

Direct Quantification of Protein–Protein Interactions in Living Bacterial Cells

Advanced Science, Volume 12, Issue 19, May 22, 2025.
Quantitative measurement of protein–protein interactions in living cells is vital for biotechnology applications. Fluorescence resonance energy transfer (FRET) signals in bacterial cells are often weak, leading to false‐positive. Quantitative FRET measurements are implemented to overcome the weak FRET signal.
Soojung Yi, Eunji Kim, Sora Yang, Gyeongmin Kim, Da‐Woon Bae, Se‐Young Son, Bo‐Gyeong Jeong, Jeong Seok Ji, Hyung Ho Lee, Ji‐Sook Hahn, Sun‐Shin Cha, Yeo Joon Yoon, Nam Ki Lee   +12 more
wiley   +1 more source

Population gene introgression and high genome plasticity for the zoonotic pathogen Streptococcus agalactiae [PDF]

, 2019
The influence that bacterial adaptation (or niche partitioning) within species has on gene spillover and transmission among bacteria populations occupying different niches is not well understood.
Abbott, Abby, Almeida, Baily, Bankevich, Beerli, Beerli, Benjamini, Bertels, Bikard, Bisharat, Bishop, Bohnsack, Borchardt, Brett M Probert, Brochet, Bruen, Brynildsrud, Capella-Gutierrez, Chen, Chen, Cheng, Chiara Crestani, Chopra, Christopher D Town, Conrad, Croucher, Da Cunha, Delannoy, Delannoy, Dogan, Edgar, Enright, Erwin, Fernandez, Ferreira, Flores, Fluegge, Garrett H Springer, Gauthier, Glazko, Glazko, Greig, Guglielmini, Gupta, Hayley B Hassler, Heaps, Holt, Imperi, Inouye, Irina M Velsko, Jafar, Jaskowiak, Jeukens, Johri, Jones, Jones, Jorgensen, Joubrel, Kalimuddin, Kim, Konig, Langdon, Librado, Lin, Lindahl, Liu, Liu, Lopez-Sanchez, Loytynoja, Lyhs, Manning, Manning, Martins, Marttinen, Mather, McArthur, Md Tauqeer Alam, Michael J Stanhope, Morse, Murrell, Page, Pal, Paulina D Pavinski Bitar, Pedersen, Petrovska, Pond, Poyart, Price, Qin, Richards, Richards, Richards, Rosinski-Chupin, Ruth N Zadoks, Sahl, Sahl, Sahl, Scheffer, Schrieber, Seemann, Shannon D Manning, Shapiro, Shepheard, Sheppard, Spoor, Springman, Srivastava, Stamatakis, Stoddard, Sukhnanand, Supek, Tettelin, Tettelin, Tian, van der Mee-Marquet, Verani, Viana, Vincent P Richards, Yu, Zadoks, Zankari, Zerbino, Zhang, Zhu   +124 more
core   +2 more sources