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Deletion of atypical type II restriction genes in Clostridium cellulovorans using a Cas9-based gene editing system. [PDF]

open access: yesAppl Microbiol Biotechnol
Schöllkopf AI   +5 more
europepmc   +1 more source
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The lac Operon

Case Studies in Systems Biology, 2021
The lac operon regulatory mechanism allows bacteria to sense external nutrient conditions and, accordingly, regulate gene transcription that assists in the digestion of available nutrients. The lac system produces a switch-like dynamic behavior, where the switching between ‘on’ and ‘off’ states of lac gene transcription depends on the specific ...
P. Kraikivski
openaire   +3 more sources

Allostery and the lac Operon

Journal of Molecular Biology, 2013
The ability to regulate gene expression is essential for controlling metabolic events in a cell. Proteins that function like molecular switches respond to fluctuations in the environment to maintain homeostasis. The operon model, proposed by Jacob and Monod, provides a cogent depiction for how gene expression is regulated. A molecular mechanism for the
M. Lewis
openaire   +4 more sources

Isolation of Pure lac Operon DNA*

Nature, 1969
Two specialized transducing phages, which carry the lac operon of Escherichia coli inserted into their DNA in opposite orientations, are used as a source of complementary sequences from which to prepare pure lac operon duplex. The availability of a single pure promoter will facilitate many new experiments on genetic transcription.
Larry Eron   +8 more
openaire   +4 more sources

Reprogramming T7RNA Polymerase in Escherichia coli Nissle 1917 under Specific Lac Operon for Efficient p-Coumaric Acid Production.

ACS Synthetic Biology, 2022
Lac operon is the standard regulator used to control the orthogonality of T7RNA polymerase (T7RNAP) and T7 promoter inEscherichia coli BL21(DE3) strain for protein expression. However,E. coliNissle 1917 (EcN), the unique probiotic strain, has seldom been
S. S. W. Effendi, I. Ng
semanticscholar   +1 more source

Modeling Gene Expression: Lac operon

2021 43rd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC), 2021
Gene regulation is an essential process for cell development, having a profound effect in dictating cell functions. Bacterial genes are often regulated through inducible systems like the Lac operon which plays an important role in cell metabolism. An accurate model of its regulation can reveal the dynamics of gene expression.
Sarai, Velazco   +6 more
openaire   +2 more sources

Hopf Bifurcation and Delay-Induced Turing Instability in a Diffusive lac Operon Model

International Journal of Bifurcation and Chaos in Applied Sciences and Engineering, 2016
In this paper, we investigate the dynamics of a lac operon model with delayed feedback and diffusion effect. If the system is without delay or the delay is small, the positive equilibrium is stable so that there are no spatial patterns formed; while the ...
Xin Cao, Yongli Song, Tonghua Zhang
semanticscholar   +1 more source

Enhancing biosynthesis of 2'‐Fucosyllactose in Escherichia coli through engineering lactose operon for lactose transport and α ‐1,2‐Fucosyltransferase for solubility

Biotechnology and Bioengineering, 2022
2'‐Fucosyllactose (2'‐FL) is the most abundant oligosaccharide in human milk and one of the most actively studied human milk oligosaccharides (HMOs). When 2'‐FL is produced through biological production using a microorganism, like Escherichia coli, d ...
B. S. Park   +7 more
semanticscholar   +1 more source

Catabolite Sensitive Site of the lac Operon

Nature, 1969
A partial deletion in the lac p region makes the lac operon insensitive to catabolite repression.
Allen E. Silverstone   +4 more
openaire   +3 more sources

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