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FrlP, an ABC type I importer component of <i>Bacillus subtilis</i>: regulation and impact in bacterial fitness. [PDF]
Gonçalves IC +3 more
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Modeling Gene Expression: Lac operon
2021 43rd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC), 2021Gene regulation is an essential process for cell development, having a profound effect in dictating cell functions. Bacterial genes are often regulated through inducible systems like the Lac operon which plays an important role in cell metabolism. An accurate model of its regulation can reveal the dynamics of gene expression.
Sarai, Velazco +6 more
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Isolation of Pure lac Operon DNA*
Nature, 1969Two specialized transducing phages, which carry the lac operon of Escherichia coli inserted into their DNA in opposite orientations, are used as a source of complementary sequences from which to prepare pure lac operon duplex. The availability of a single pure promoter will facilitate many new experiments on genetic transcription.
J, Shapiro +5 more
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2021
The lac operon regulatory mechanism allows bacteria to sense external nutrient conditions and, accordingly, regulate gene transcription that assists in the digestion of available nutrients. The lac system produces a switch-like dynamic behavior, where the switching between ‘on’ and ‘off’ states of lac gene transcription depends on the specific ...
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The lac operon regulatory mechanism allows bacteria to sense external nutrient conditions and, accordingly, regulate gene transcription that assists in the digestion of available nutrients. The lac system produces a switch-like dynamic behavior, where the switching between ‘on’ and ‘off’ states of lac gene transcription depends on the specific ...
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Catabolite Sensitive Site of the lac Operon
Nature, 1969A partial deletion in the lac p region makes the lac operon insensitive to catabolite repression.
A E, Silverstone +4 more
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Journal of Molecular Biology, 2013
The ability to regulate gene expression is essential for controlling metabolic events in a cell. Proteins that function like molecular switches respond to fluctuations in the environment to maintain homeostasis. The operon model, proposed by Jacob and Monod, provides a cogent depiction for how gene expression is regulated. A molecular mechanism for the
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The ability to regulate gene expression is essential for controlling metabolic events in a cell. Proteins that function like molecular switches respond to fluctuations in the environment to maintain homeostasis. The operon model, proposed by Jacob and Monod, provides a cogent depiction for how gene expression is regulated. A molecular mechanism for the
openaire +2 more sources
Transient Repression of the lac Operon
Journal of Bacteriology, 1967Severe transient repression of constitutive or induced β-galactosidase synthesis occurs upon the addition of glucose to cells of Escherichia coli growing on glycerol, succinic acid, or lactic acid. Only mutants particularily well adapted to growth on glucose exhibit this phenomenon when transferred to a glucose ...
B, Tyler, W F, Loomis, B, Magasanik
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Cost-Benefit Tradeoffs in Engineered lac Operons
Science, 2012Accounting for Lac When Escherichia coli expresses the lac operon, it needs to balance the potential increase in growth rate conferred through having the encoded proteins ...
Matt, Eames, Tanja, Kortemme
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Genetic fusions defining trp and lac operon regulatory elements
Journal of Molecular Biology, 1975Abstract Two procedures for easily isolating deletions that fuse the trp and lac operons are described. Using these procedures, a large number of fusion deletions have been isolated. The lac ends of these deletions extend varying distances into the lacI gene and the lac promoter-operator region.
D H, Mitchell +2 more
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A complex educational module designed to evaluate students’ capacity in applying knowledge of mutations within the lactose operon system of bacteria. Through this module, students will gain proficiency in correlating mutations with genotypes to make predictions about phenotypes in diverse environmental conditions.
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