Machine Learning Prediction of Laccase-Catalyzed Oxidation of Aromatic Compounds Using Curated Enzyme-Specific Datasets. [PDF]
J Comput ChemWe curate laccase‐substrate datasets and train five classifiers, from regularized logistic regression to tree‐based models and ChemBERTa, to predict whether a substrate will be oxidized. Feature importance and attention maps projected onto molecular substructures make the predictions interpretable and useful for pre‐screening before the bench ...
Kulagina Y +3 more
europepmc +2 more sources
Enzymatic Characteristics of Laccase from Lactobacillus plantarum and Its Effect on Degradation of Biogenic Amines [PDF]
Shipin Kexue, 2023 In order to study the enzymatic properties of laccase derived from Lactobacillus and its potential application in biogenic amine degradation, the gene encoding laccase from Lactobacillus plantarum was heterologously expressed and the recombinant laccase ...
CAO Yuying, WANG Shuang, LIU Yining, GAO Manman, DU Liping, MA Lijuan, ZHANG Huiling, TIAN Xiaoju, YANG Weiming
doaj +1 more source
A la carte bioprospecting of substrate-selective laccases via high-throughput computational enzyme-substrate interaction profiling. [PDF]
Protein SciAbstract Laccases are versatile, multicopper oxidases with broad biotechnological applications due to their ability to oxidize a wide range of substrates. Here, we introduce a computational pipeline that combines sequence filtering, Monte Carlo simulations, and quantum mechanical spin density calculations to identify laccase candidates tailored to ...
Xiang R +5 more
europepmc +2 more sources
Potential Degradation and Kinetics of Melanoidin by Using Laccase from White Rot Fungus
Applied Environmental Research, 2020 This study was attempted to use laccase extracted from white rot fungus to remove melanoidin in the ethanol production wastewater. The isolated fungus producing the highest laccase was identified as Megaspororia sp.
Wittawat Toomsan +4 more
doaj +7 more sources
Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase [PDF]
Journal of the Serbian Chemical Society, 2018 The biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soils by laccase is reported. However, the low laccase activities of free laccase have limited its applications in environmental bioremediation. In this study, polluted soil was made with 3:
Wang Xin +4 more
doaj +1 more source
Laccase activity tests and laccase inhibitors
Journal of Biotechnology, 2000 Sulfhydryl organic compounds described as laccase inhibitors: dithiothreitol, thioglycolic acid, cysteine, diethyldithiocarbamic acid, and sodium azide were tested for their activity toward laccase of Trametes versicolor in different test systems utilising 2, 2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2, 6-dimethoxyphenol as ...
Johannes, C., Majcherczyk, Andrzej
openaire +3 more sources
Fungal solid state fermentation on agro-industrial wastes for acid wastewater decolourization in a continuous flow packed-bed bioreactor [PDF]
, 2011 This study was aimed at developing a process of solid state fermentation (SSF) with the fungi Pleurotus ostreatus and Trametes versicolor on apple processing residues for wastewater decolorization.
AMORE, ANTONELLA +5 more
core +1 more source
Biotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture [PDF]
تحقیقات علوم چوب و کاغذ ایران, 2012 Enzymatic activation of lignin on pinewood TMP (Thermo-Mechanical Pulp) fibers for MDF production was investigated using electronic microscopic technique. Two systems: laccase mediator and laccase without mediator were used.
Alireza Kharazipoor
doaj +1 more source
Repurposing designed mutants: a valuable strategy for computer-aided laccase engineering – the case of POXA1b [PDF]
, 2017 The broad specificity of laccases, a direct consequence of their shallow binding site, makes this class of enzymes a suitable template to build specificity toward putative substrates.
Giacobelli, Valerio G. +6 more
core +2 more sources
Laccase immobilized on magnetic nanoparticles by dopamine polymerization for 4-chlorophenol removal
Green Energy & Environment, 2017 In this work, a new immobilization method based on dopamine (DA) self-polymerization was developed for laccase immobilization on magnetic nanoparticles (Fe3O4 NPs).
Di Zhang +4 more
doaj +1 more source