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Human lactase and the molecular basis of lactase persistence

Biochemical Genetics, 1985
Human lactase purified from detergent extracts of the total membrane fraction of postmortem jejunum by means of monoclonal immunoadsorbent chromatography appears to be a dimer of subunits identical in Mr (160K). Trypsin or papain removes a small hydrophobic anchoring peptide from each subunit to give a hydrophilic enzyme which no longer interacts with ...
Anna J. Furth   +5 more
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Lactase Insufficiency Revisited

Journal of Pediatric Gastroenterology and Nutrition, 1985
The definition of “insufficient” small bowel lactase activity varies greatly among authors. The present study is aimed at redefining lactase insufficiency by comparing intestinal lactase activity and results of the lactose breath hydrogen test. Primary “insufficient” lactase activity was considered to be present when a child with a normal small bowel ...
P Forget   +4 more
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Lactase Deficiency in Orientals

Gastroenterology, 1968
Summary In our study all 11 healthy Orientals were shown to have isolated small bowel lactase deficiency. Ten Orientals had symptoms after ingesting 50 g of lactose but tolerated modest amounts of milk.
Myung H. Chung, Douglas B. McGill
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Immunocytochemical heterogeneity of lactase-phlorizin hydrolase in adult lactase deficiency [PDF]

open access: possibleGastroenterology, 1993
Adult lactase deficiency may result either from diminished synthesis of precursor lactase-phlorizin hydrolase (phenotype I deficiency) or from altered posttranslational processing of the precursor protein (phenotype II). The aim of this study was to compare the location of lactase-phlorizin hydrolase along the crypt-villus axis in control with adult ...
Ward A. Olsen   +2 more
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Occupational sensitization to lactase

American Journal of Industrial Medicine, 1997
Occupational sensitization to lactase is reported in workers formulating and packaging this consumer product, which is used for the relief of gastrointestinal symptoms caused by intolerance to lactose. Allergic rhinitis, conjunctivitis, and some cases of asthma were noted.
Jim A. Julian   +5 more
openaire   +2 more sources

Lactase activity of microorganisms

Folia Microbiologica, 1978
Sixty-two strains of yeasts, molds and bacteria were screened for lactase (beta-D-galactosidase) activity. Strains exhibiting the enzyme activity were evaluated for cell yield as well as enzyme units available per litre of the medium, per g cell dry weight and per mg protein of their cell-free extracts.
S. M. Dutta, M. V. R. Rao
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Purified lactases versus whole-cell lactases—the winner takes it all

Applied Microbiology and Biotechnology, 2021
Lactose-free dairy products are in great demand worldwide due to the high prevalence of lactose intolerance. To make lactose-free dairy products, commercially available β-galactosidase enzymes, also termed lactases, are used to break down lactose to its constituent monosaccharides, glucose and galactose.
Robin Dorau   +2 more
openaire   +3 more sources

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