Results 131 to 140 of about 382,242 (307)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Nouvelle série électronique e-LAM

, 2014
Le LAM (Les Afriques dans le Monde, Sciences Po Bordeaux) lance une série électronique dite e-LAM. Voici la présentation que les responsables de la série, Michel Cahen et Vincent Foucher, en ont faite : e-LAM  Concepts, méthodologie et controverses La ...
Camille Al Dabaghy
core   +1 more source

Using ultrasonography in observation of the gonadal development of striped catfish (Pangasianodon hypophthalmus)

Fisheries and Aquatic Sciences
Ultrasound images can be utilized as a non-invasive method in the reproductive program. Striped catfish in various gonadal development were examined with a portable Mindray Model Z60Vet using the Convex probe at a frequency of 5 MHz.
Hoa Phu Nguyen, Trong Thanh Tran, Hien Thi Thanh Nguyen, Linh Ngoc Thuy Bui, Luong Cong Trung   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Mortality by LAM result.

, 2013
Mortality by LAM result.
Muhammad Bakari (316249), Elizabeth Talbot (330185), Pedro Teixeira (330190), Patricia Munseri (330188), Timothy Lahey (320330), Mecky Matee (66135), Fordham von Reyn (330192)   +6 more
core   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Evaluation and validation of reference genes for RT-qPCR normalization in different sweet potato tissues

Scientific Reports
Sweet potato (Ipomoea batatas) is one of the most important crops of the world, displaying a significant economic importance in several countries. However, few studies exploring biotechnological tools, such as RT-qPCR, used for gene expression analysis ...
Melissa Barbosa Fonseca Moraes, Matheus Martins Daúde, Kellen Kauanne Pimenta de Oliveira, Rogério Cavalcante Gonçalves, Solange Aparecida Ságio, André Almeida Lima, Antonio Chalfun-Junior, Márcio Antônio da Silveira, Horllys Gomes Barreto   +8 more
doaj   +1 more source

Oral History of Cathy Lam

, 2013
This is an oral history of Ms. Cathy Lam. She is a graduate of the University of California, Irvine. She graduated college early with an engineering degree. Upon graduating, she pursued a career with an engineering company but later decided to change her
Lam, Cathy
core  

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source