Results 131 to 140 of about 2,779,394 (268)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Objective quality assessment for precision functional MRI data. [PDF]

Neuron
Lynch CJ, Chang M, Elbau I, Gordon EM, Laumann TO, Du J, Ladwig Z, Lueckel M, Perez DC, Summerville I, Chou J, Johnson M, Ho C, Manfredi N, Nilchian P, Solomonov N, Goldwaser E, Ng T, Moia S, Caballero-Gaudes C, Downar J, Vila-Rodriguez F, Gregory E, Daskalakis ZJ, Blumberger DM, Kay K, Buchanan DM, Williams N, Bhati MT, Clauss J, Zebley B, Victoria LW, Power JD, Grosenick L, Gunning FM, Liston C.   +35 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Lysozyme revisited: evaluating models of a reference protein in structural biology. [PDF]

Curr Res Struct Biol
Wlodawer A, Rubach P, Dauter Z, Dec W, Brzezinski D, Jaskolski M, Minor W.   +6 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Direct demonstration of electric chirality control in a helimagnetic YMn₆Sn₆ by spin-polarized neutron scattering. [PDF]

Proc Natl Acad Sci U S A
Masuda H, Yanagisawa Y, Ohishi K, Nambu Y, Nii Y, Onose Y.   +5 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Structure-function coupling of large-scale cortical networks across the lifespan is spectrally specific. [PDF]

Commun Biol
Picci G, Rempe MP, Petro NM, Son JJ, Casagrande CC, Ott LR, Penhale SH, Embury CM, Schantell M, Johnson HJ, Okelberry HJ, Willett MP, Wang YP, Stephen JM, Calhoun VD, Wiesman AI, Kurz MJ, Heinrichs-Graham E, Wilson TW.   +18 more
europepmc   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source