Results 51 to 60 of about 205,872 (218)

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

open access: yesFEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Editorial: Advances and applications of laser-material interaction

open access: yesFrontiers in Physics, 2023
Kamran Avanaki   +6 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

open access: yesFEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

Numerical Investigation of Enhanced High-Intensity Laser–Matter Interactions in Nanowire-Coated Conical Targets

open access: yesNanomaterials
Nanostructured targets are increasingly used as key components in high-power laser–matter interaction experiments due to their ability to substantially enhance laser absorption, increase ion/electron generation, or boost the secondary radiation (THz, X ...
Laura Ionel, Cristian Viespe
doaj   +1 more source

Peripheral Neutrophil Activation and Extracellular Trap Formation in Amyotrophic Lateral Sclerosis

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
Markers of neutrophil activation are increased in plasma during ALS, and markers of NET formation associate with ALS survival. ABSTRACT Objectives Peripheral neutrophil levels in amyotrophic lateral sclerosis (ALS) inversely correlate with survival, suggesting a role for neutrophils in disease progression.
Lillia A. Baird   +9 more
wiley   +1 more source

PHYSICAL SEQUENTIAL MODELS FOR LASER CUTTING

open access: yesNonconventional Technologies Review, 2016
Laser cutting of composite materials requires improving physical models applied to laser cutting process. In setting of machining parameters knowledge of physical phenomena occurring in the material plays important role.
Remus Boboescu, Richard Herman
doaj  

Material-specific beam-plume interactions during deep-penetration laser welding of stainless steel, aluminum, and copper

open access: yesJournal of Advanced Joining Processes
During deep-penetration laser welding, a hot vapor plume is emitted from the keyhole which, on cooling, condenses into a particle cloud that surrounds the weld zone.
Johannes Wahl   +7 more
doaj   +1 more source

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