Results 61 to 70 of about 1,205,027 (295)
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
High-average-power narrow-linewidth tunable solid-state lasers in the wavelength region between 2 and 3 μm are attractive light sources for many applications.
Oleg Antipov +6 more
doaj +1 more source
Investigations were carried out at the multistage hybrid Ti:sapphire–KrF laser facility GARPUN-MTW on the direct amplification of TW-power picosecond UV laser pulses in e-beam-pumped KrF amplifiers and propagation along a 100 m laboratory air pass.
V. D. Zvorykin +2 more
doaj +1 more source
The effect of tens-of-Watt seed light on the stimulated Raman scattering (SRS) in a distributed side-pumped Yb-doped fiber amplifier is studied. By using two sorts of seed sources with the power smaller than 70 W, it is observed in experiment that the ...
Heng Chen +7 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Threonine 348 regulates the subcellular localization of PTEN
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley +1 more source
Predicting extreme defects in additive manufacturing remains a key challenge limiting its structural reliability. This study proposes a statistical framework that integrates Extreme Value Theory with advanced process indicators to explore defect–process relationships and improve the estimation of critical defect sizes. The approach provides a basis for
Muhammad Muteeb Butt +8 more
wiley +1 more source
This article presents the design, modeling, and characterization of air‐pressure–actuated programmable vibroacoustic metamaterials (PVAMM). The study focuses on leveraging air pressure to dynamically tune resonance frequencies for effective noise attenuation.
William Kaal +2 more
wiley +1 more source
A study on the amplification of active-mirror Yb:YAG lasers
Thermal effects and amplified spontaneous emission (ASE) are two main factors in the design of high-power and high-energy laser amplifiers. The temperature distribution and the lateral ASE of the face-pumped active-mirror Yb:YAG amplifiers are analyzed ...
Xiaojin Cheng +3 more
doaj

