Results 31 to 40 of about 8,680 (259)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Manufacturing of Porous Glass by Femtosecond Laser Welding
Micromachines, 2022 Based on femtosecond laser glass welding, four different porous structures of welding spots were formed by the manufacturing processes of spatiotemporal beam shaping and alternating high repetition rate transformation.
Hua Tan +6 more
doaj +1 more source
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Wavelength-independent laser beam shaping [PDF]
SPIE Proceedings, 2010 This paper presents a beam shaping device namely, a Diffractive Optical Element (DOE), which is used to change a beam having a Gaussian intensity profile into a beam with a uniform intensity profile. The DOE used in this work was fabricated from ZnSe and its performance was evaluated using a cw CO 2 laser.
Mapule P. Degama, Andrew Forbes
openaire +1 more source
Advanced Engineering Materials, EarlyView.Predicting extreme defects in additive manufacturing remains a key challenge limiting its structural reliability. This study proposes a statistical framework that integrates Extreme Value Theory with advanced process indicators to explore defect–process relationships and improve the estimation of critical defect sizes. The approach provides a basis for
Muhammad Muteeb Butt +8 more
wiley +1 more source
Applied Sciences, 2020 The latest research on laser beam fusion cutting (LBFC) with static beam shaping have shown a limitation in the quality of cut parts for thick steel plates (> 6 mm) when using solid state lasers.
Cindy Goppold +4 more
doaj +1 more source
Results in Optics, 2021 The use of pulsed high-power diode lasers (HPDLs) in the near infrared (NIR) range as multispectral sources has attracted much interest in biomedical applications due to their relatively low cost and small size compared to nanosecond Nd:YAG optical ...
L. Leggio +4 more
doaj +1 more source
Advanced Engineering Materials, EarlyView.Thermomechanical fatigue tests of laser beam powder bed fusion (PBF‐LB) Inconel 718 show that the additively manufactured material reaches almost the lifetimes of conventionally‐rolled material under no‐dwell conditions. Introducing dwell times at the maximum temperature markedly reduces the lifetimes due to pronounced grain boundary sliding associated
Stefan Guth +6 more
wiley +1 more source