Results 61 to 70 of about 2,890,960 (258)

From quantum heat engines to laser cooling: Floquet theory beyond the Born–Markov approximation [PDF]

New Journal of Physics, 2017
We combine the formalisms of Floquet theory and full counting statistics with a Markovian embedding strategy to access the dynamics and thermodynamics of a periodically driven thermal machine beyond the conventional Born–Markov approximation. The working
S. Restrepo, J. Cerrillo, P. Strasberg, G. Schaller   +3 more
semanticscholar   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

2D transverse laser cooling of a hexapole focused beam of cold BaF molecules

Communications Physics
Laser cooling of molecules is a powerful technique for producing cold, slow beams for precision measurements and quantum control, yet its implementation remains challenging due to molecular complexity.
J. W. F. van Hofslot, I. E. Thompson, A. Touwen, N. Balasubramanian, R. Bause, H. L. Bethlem, A. Borschevsky, T. H. Fikkers, S. Hoekstra, S. A. Jones, J. E. J. Levenga, M. C. Mooij, H. Mulder, B. A. Nijman, E. H. Prinsen, B. J. Schellenberg, L. van Sloten, R. G. E. Timmermans, W. Ubachs, J. de Vries, L. Willmann, On behalf of the NL-eEDM collaboration   +21 more
doaj   +1 more source

Progress in laser cooling semiconductor nanocrystals and nanostructures

NPG Asia Materials, 2019
Over the past two decades, there have been sizable efforts to realize condensed phase optical cooling. To date, however, there have been no verifiable demonstrations of semiconductor-based laser cooling.
Shubin Zhang, M. Zhukovskyi, B. Jankó, M. Kuno   +3 more
semanticscholar   +1 more source

Thermomechanical Fatigue and Creep–Fatigue Interaction of Inconel 718 Additively Manufactured by Laser Beam Powder Bed Fusion

Advanced Engineering Materials, EarlyView.
Thermomechanical fatigue tests of laser beam powder bed fusion (PBF‐LB) Inconel 718 show that the additively manufactured material reaches almost the lifetimes of conventionally‐rolled material under no‐dwell conditions. Introducing dwell times at the maximum temperature markedly reduces the lifetimes due to pronounced grain boundary sliding associated
Stefan Guth, Tomáš Babinský, Jan Lars Riedel, Wen Hao Kan, Aijun Huang, Martin Heilmaier, Ivo Šulák   +6 more
wiley   +1 more source

Disk Laser Welding of Car Body Zinc Coated Steel Sheets / Spawanie Laserem Dyskowym Blach Ze Stali Karoseryjnej Ocynkowanej

Archives of Metallurgy and Materials, 2015
Autogenous laser welding of 0.8 mm thick butt joints of car body electro-galvanized steel sheet DC04 was investigated. The Yb:YAG disk laser TruDisk 3302 with the beam spot diameter of 200 μm was used.
Lisiecki A., Burdzik R., Siwiec G., Konieczny Ł., Warczek J., Folęga P., Oleksiak B.   +6 more
doaj   +1 more source

Low‐Angle Grain Boundaries and Re‐Segregation in Single‐Crystalline Ni‐Base Superalloys

Advanced Engineering Materials, EarlyView.
This work demonstrates that Re‐segregation at low‐angle grain boundaries (LAGBs) in Ni‐base superalloys is influenced by misorientation angle. Advanced microscopy and atom probe tomography reveal that higher misorientation angles increases Re‐segregation.
Alireza B. Parsa, Aparna Saksena, Alesander Kostka, Pascal Thome, Felicitas Scholz, Alexandre Mussi, Christoph Somsen, Baptiste Gault, Jan Frenzel, Gunther Eggeler   +9 more
wiley   +1 more source

The Effect of Cooling Layer Thickness and Coolant Velocity on Crystal Thermodynamic Properties in a Laser Amplifier

Micromachines, 2023
Laser diode pumped solid-state lasers (DPSSLs) have been widely used in many fields, and their thermal effects have attracted more and more attention. The laser diode (LD) side-pumped amplifier, as a key component of DPSSLs, is necessary for effective ...
Shuzhen Nie, Tianzhuo Zhao, Xiaolong Liu, Pubo Qu, Yuchuan Yang, Yuheng Wang   +5 more
doaj   +1 more source