Results 51 to 60 of about 5,055,638 (241)

Laser-Induced Fluorescence Spectroscopy in the Diagnosis of Tissue Hypoxia (Review)

Общая реаниматология, 2019
The aim of review is to discuss the results of studies on diagnosis of tissue hypoxia by laser-induced spectroscopy, as well as to identify promising trends and prospects of this technique for its further application in experimental and clinical medicine.
Anastasiya S. Babkina
doaj   +1 more source

Paclitaxel induces NM2‐dependent cellular contraction through GEF‐H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells

Molecular Oncology, EarlyView.
Taxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Gloria Asensio‐Juárez, Rafael Pérez‐Díaz, Hugo Ramos‐Solano, Marina Garrido‐Casado, Vanessa C. Talayero, Miguel Vicente‐Manzanares   +5 more
wiley   +1 more source

Kinetics of 5-aminolevulinic acid-induced fluorescence in organ cultures of bronchial epithelium and tumor [PDF]

, 2002
Background: 5-Aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PPIX) fluorescence improves the differentiation of tumor and normal tissue in the bladder, skin and brain. Objective: The kinetics of 5-ALA-induced protoporphyrin IX (PPIX) fluorescence
Bergner, Albrecht, Maier, Irmgard, Gamarra, Fernando, Al-Batran, Salah, Baumgartner, Reinhold, Wagner, Simon, Castro, Mirna, Hautmann, Hubert, Huber, Rudolf M.   +8 more
core   +1 more source

p190A/ARHGAP35 and p190B/ARHGAP5 proteins in endometrial cancer: a novel cancer‐relevant paralog interplay

Molecular Oncology, EarlyView.
This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault, Capucine Heraud, Mélissa Correia de Oliveira, Véronique Neaud, Valérie Valesco, Valérie Prouzet‐Mauleon, Béatrice Turcq, Jean‐William Dupuy, Anne‐Aurélie Raymond, Sabrina Croce, Frédéric Saltel, Valérie Lagrée, Violaine Moreau   +12 more
wiley   +1 more source

A real time reference system for wavelength locking in laser induced fluorescence with gas expansion (LIF-FAGE) measurement of atmospheric hydroxyl (OH) radicals [PDF]

Atmospheric Measurement Techniques
The hydroxyl radical (OH) plays a central role in atmospheric chemistry, however, its accurate measurement by laser induced fluorescence with gas expansion (LIF-FAGE) is unavoidably compromised by wavelength drift of the excitation laser.
S. Chen, C. Zhang, Y. Wang, Q. Zang, X. Ma, Z. Tan, L. Zeng, Y. Zhang, K. Lu   +8 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Effect of Absorption and Scattering on Fluorescence of Buried Tumours

Journal of Spectroscopy, 2020
Fluorescence spectroscopy is widely used for biomedical optical diagnosis and surgical resection of tumours. This work investigates laser-induced fluorescence spectroscopy of fluorescence inclusions that are embedded in turbid media.
Kawthar M. K. Alghourani, Wesam Bachir, George Karraz   +2 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Active 3D Imaging of Vegetation Based on Multi-Wavelength Fluorescence LiDAR

Sensors, 2020
Comprehensive and accurate vegetation monitoring is required in forestry and agricultural applications. The optical remote sensing method could be a solution.
Xingmin Zhao, Shuo Shi, Jian Yang, Wei Gong, Jia Sun, Biwu Chen, Kuanghui Guo, Bowen Chen   +7 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source