Results 61 to 70 of about 5,055,638 (241)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Diagnostic for the characterization of nanometric structures in high temperature reactive systems [PDF]

, 2008
It is now well know that the fraction of particulate mater in the ambient air defined as ultrafine particles can be considered the most critical for adverse human health effects because of their chemical composition and the ability of these particles to ...
Commodo, Mario
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Fluorescence diagnostics of dental caries

Доклады Белорусского государственного университета информатики и радиоэлектроники, 2019
The results obtained in studies of laser-induced fluorescence of the normal and carries-affected dental tissues are presented. The possibility of detection of considerable depth caries with use of the specially designed spectrometric system with ...
L. S. Lyashenko, M. P. Samtsov, E. S. Voropay, A. V. Butvilovski, V. R. Gaifullina, M. V. Bobkova   +5 more
doaj  

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

LIF measurement in a partially saturated and partially absorbed regime

Frontiers in Physics
The problems of laser-induced fluorescence (LIF) measurements in a partially saturated regime with spatially dependent laser intensity in the sample (caused by absorption) are analyzed.
Pavel Dvořák, Martina Mrkvičková, Jan Kratzer   +2 more
doaj   +1 more source

Second-derivative laser-induced fluorescence spectroscopy combined with chemometrics for authentication of the adulteration of camellia oil

CyTA - Journal of Food, 2018
In this study, second-derivative laser-induced fluorescence spectroscopy (SD-LIF) and its derivative intrinsic-ratiometric laser-induced fluorescence spectroscopy (IR-LIF) were developed for the qualitative and quantitative analysis of camellia oil ...
Hui Chen, Dechun Geng, Tong Chen, Daoli Lu, Bin Chen   +4 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Early screening of dentin caries using the methods of Micro-Raman and laser-induced fluorescence spectroscopy

Results in Physics, 2018
The work presents microspectroscopic studies of the caries development and phase formation in dentin tissues using the methods of laser-induced fluorescence (LIF) and Raman spectroscopy. The results show that the data obtained near the boundary of intact
D.L. Goloshchapov, V.M. Kashkarov, Y.A. Ippolitov, T. Prutskij, P.V. Seredin   +4 more
doaj   +1 more source