Results 41 to 50 of about 6,304,985 (297)

Linewidth and phase noise characteristics of DFB fibre lasers

open access: yes, 2004
The anomalous linewidth behavior in a DFB fiber laser is investigated. It is shown that not only does the linewidth deviate drastically from the Schawlow-Townes linewidth formula by increasing with pump and laser power, but it also varies significantly ...
Voo, N.Y.   +7 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Stable 100 GHz Pulses Generated by injection locking of multiple lasers to an optical frequency comb

open access: yes, 2013
Optical frequency combs (OFCs) can generate a wide bandwidth of regularly spaced modes with high frequency stability. Besides frequency and time metrology, OFCs can also have applications in arbitrary waveform generation and telecommunications by ...
Wu, D.S.   +5 more
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Near ultraviolet photonic integrated lasers based on silicon nitride

open access: yesAPL Photonics, 2022
Low phase noise lasers based on the combination of III–V semiconductors and silicon photonics are well established in the near-infrared spectral regime.
Anat Siddharth   +12 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Laser Heterodyne Detection Based on Photon Time–Domain Differential Detection Avoiding the Effect of Decoherence Phase Noise

open access: yesSensors, 2023
Laser heterodyne detection (LHD) is a key velocimetry technique that provides better accuracy and sensitivity than direct laser detection. However, random phase noise can be introduced by the surface topography of the moving target undulation or ...
Ce Guan   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Sub-60-fs Timing Jitter of a SESAM Modelocked VECSEL

open access: yesIEEE Photonics Journal, 2013
We present noise measurements of a pulse train emitted from an actively stabilized semiconductor-saturable-absorber-mirror (SESAM) modelocked vertical external cavity surface emitting laser (VECSEL).
V. J. Wittwer   +6 more
doaj   +1 more source

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