Results 171 to 180 of about 1,086 (217)
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A UV laser-scanning confocal microscope for the measurement of intracellular Ca2+
Cell Calcium, 1994Modifications to the optics of a conventional confocal laser-scanning microscope were made to allow imaging intracellular Ca(2+)-dependent fluorescence with a UV laser (351 or 364 nm). Modifications included: (1) a chromatic compensation lens in the laser path; (2) the design of a practically achromatic relay lens; (3) a longer tube length for the ...
K, Kuba, S Y, Hua, T, Hayashi
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The Intermediate Optical System of Laser-scanning Confocal Microscopes
1990This text explains some of the basics of intermediate optical systems in confocal microscopes. Important microscope components are discussed in more detail in other chapters: lasers (Chapter 5) and other light sources (Chapter 6), objective lenses (Chapter 7), special optical elements (Chapter 3), fibers (Chapter 26), and detectors (Chapter 12 ...
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A custom confocal and two-photon digital laser scanning microscope
American Journal of Physiology-Heart and Circulatory Physiology, 2000We describe a custom one-photon (confocal) and two-photon all-digital (photon counting) laser scanning microscope. The confocal component uses two avalanche photodiodes (APDs) as the fluorescence detector to achieve high sensitivity and to overcome the limited photon counting rate of a single APD (∼5 MHz).
W G, Wier +3 more
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Spherical-coordinate scanning confocal laser microscope
Optical Engineering, 1995We present the design, construction, and characterization of a spherical-coordinate scanning, reflection-mode confocal laser microscope (SCSLM). Two system designs are presented, namely a conventional bulk optics object-scanning design and a preliminary optical-fiber-based two-axis optics/one-axis object scanning version.
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Three-dimensional microscopy using a confocal laser scanning microscope
Optics Letters, 1985In a scanning laser microscope detecting fluorescent light from the specimen, the depth-discriminating property of confocal scanning has been used to carry out optical slicing of a thick specimen. The recorded digital images constitute a three-dimensional raster covering a volume of the specimen.
K, Carlsson +5 more
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A video rate confocal laser scanning microscope
Optical Society of America Annual Meeting, 1992A video rate confocal laser scanning microscope (CLSM) for biological applications has been developed for manufacture. This microscope is used for viewing thin "optical sections" of a sample by rejecting the signal from out-of-focus planes. The microscope consists of a multiline argon ion laser, a galvanometer and acousto-optic deflector (AOD) for ...
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Ultrastructural and confocal laser scanning microscopic examination of TUNEL‐positive cells
The Journal of Pathology, 1997TdT-mediated dUTP-biotin nick end labelling (TUNEL) has been widely used for detecting cells with DNA fragmentation or apoptotic cells. However, since the concept of apoptosis is based on cellular ultrastructure, it is important to identify the morphological features of TUNEL-positive cells.
K, Kimura +9 more
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Scanning and detection techniques used in a confocal scanning laser microscope
Journal of Microscopy, 1990SUMMARYA two‐mirror scanning mechanism for confocal microscopy is described. No optical components, in addition to the scanning mirrors, are used. Design criteria and performance of the scanner are discussed. The photometric linearity of a detector unit incorporating a photomultiplier tube is reported, and a dual detector unit with tunable split ...
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Optically Corrected Diode Laser for Confocal Scanning Microscope
Frontiers in Optics 2007/Laser Science XXIII/Organic Materials and Devices for Displays and Energy Conversion, 2007We propose the use of optically corrected laser diode in the confocal scanning microscope. The resulted beam has no astigmatism and produces a round diffraction limited spot on a sample.
Dmitry Rozhetskin +2 more
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Near-Infrared Confocal Laser Scanning Microscope for the Analysis of Paintings
2008We designed and built a fiber optic confocal laser-scanning interference microscope operating in the NIR for the analysis of paintings. Instrumentation and preliminary results.
Pezzati L +3 more
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