Results 181 to 190 of about 255,657 (229)

Novel Micro Scanning with Integrated Atomic Force Microscope and Confocal Laser Scanning Microscope

2019 IEEE Conference on Control Technology and Applications (CCTA), 2019
Integrated atomic force microscope (AFM) and confocal laser scanning microscope (CLSM) can quickly obtain the three-dimensional (3-D) surface of the sample in large scanning range and recover the region of interesting (ROI) in nanoscale resolution. However, the traditional cooperative algorithm for integrated microscopes occupies too much scanning time.
Meng-Hao Chou, Ching-Chi Huang, Yi-Lin Liu, Huang-Chih Chen, Li-Chen Fu   +4 more
openaire   +2 more sources

Diagnostic accuracy of a new ex vivo confocal laser scanning microscope compared to H&E‐stained paraffin slides for micrographic surgery of basal cell carcinoma

Journal of the European Academy of Dermatology and Venereology, 2018
For safe excision of malignant skin tumours, complete negative surgical margins are mandatory. The gold standard for analysis is frozen sections or paraffin‐embedded haematoxylin and eosin (H&E)‐stained slides.
N. Peters, M. Schubert, G. Metzler, J.-P. Geppert, M. Moehrle   +4 more
semanticscholar   +1 more source

Dual-mode phase and fluorescence imaging with a confocal laser scanning microscope.

Optics Letters, 2018
We present dual-mode phase and fluorescence imaging in a confocal laser scanning microscopy (CLSM) system. For phase imaging, the depth of field of the CLSM system is extended by fast axial scanning with a tunable acoustic gradient index of refraction ...
Juan-Juan Zheng, Chao Zuo, Peng Gao, G. U. Nienhaus   +3 more
semanticscholar   +1 more source

Variable-ambient scanning stage for a laser scanning confocal microscope

Review of Scientific Instruments, 2003
A variable-ambient scanning stage for a laser scanning confocal microscope was designed and tested. The stage allows for facile observation of both thin films and single molecule samples under inert conditions. High precision images have been acquired up to 174 μm2 using a single piezoceramic tube.
D. J. Sirbuly, J. P. Schmidt, M. D. Mason, M. A. Summers, S. K. Buratto   +4 more
openaire   +1 more source

A custom confocal and two-photon digital laser scanning microscope

American Journal of Physiology-Heart and Circulatory Physiology, 2000
We describe a custom one-photon (confocal) and two-photon all-digital (photon counting) laser scanning microscope. The confocal component uses two avalanche photodiodes (APDs) as the fluorescence detector to achieve high sensitivity and to overcome the limited photon counting rate of a single APD (∼5 MHz).
W G, Wier, C W, Balke, J A, Michael, J R, Mauban   +3 more
openaire   +2 more sources

A Confocal Laser Scanning Microscope Investigation of the Epiphany Obturation System

Journal of Endodontics, 2007
The purpose of this study was to assess the sealer-dentin interface and compare the percentage and average depth of dentinal tubule sealer penetration in the coronal, middle and apical thirds of teeth obturated with the Epiphany Obturation System using 10x and 40x confocal laser scanning microscopy.
Saman R, Gharib, Patricia A, Tordik, Glen M, Imamura, Thomas A, Baginski, Gary G, Goodell   +4 more
openaire   +2 more sources

A video rate confocal laser scanning microscope

Optical Society of America Annual Meeting, 1992
A video rate confocal laser scanning microscope (CLSM) for biological applications has been developed for manufacture. This microscope is used for viewing thin "optical sections" of a sample by rejecting the signal from out-of-focus planes. The microscope consists of a multiline argon ion laser, a galvanometer and acousto-optic deflector (AOD) for ...
openaire   +1 more source

Three-dimensional microscopy using a confocal laser scanning microscope

Optics Letters, 1985
In a scanning laser microscope detecting fluorescent light from the specimen, the depth-discriminating property of confocal scanning has been used to carry out optical slicing of a thick specimen. The recorded digital images constitute a three-dimensional raster covering a volume of the specimen.
K, Carlsson, P E, Danielsson, R, Lenz, A, Liljeborg, L, Majlöf, N, Aslund   +5 more
openaire   +2 more sources

The Intermediate Optical System of Laser-scanning Confocal Microscopes

1990
This text explains some of the basics of intermediate optical systems in confocal microscopes. Important microscope components are discussed in more detail in other chapters: lasers (Chapter 5) and other light sources (Chapter 6), objective lenses (Chapter 7), special optical elements (Chapter 3), fibers (Chapter 26), and detectors (Chapter 12 ...
openaire   +1 more source

Spherical-coordinate scanning confocal laser microscope

Optical Engineering, 1995
We present the design, construction, and characterization of a spherical-coordinate scanning, reflection-mode confocal laser microscope (SCSLM). Two system designs are presented, namely a conventional bulk optics object-scanning design and a preliminary optical-fiber-based two-axis optics/one-axis object scanning version.
openaire   +1 more source