Results 221 to 230 of about 180,806 (262)
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A custom confocal and two-photon digital laser scanning microscope
American Journal of Physiology-Heart and Circulatory Physiology, 2000We describe a custom one-photon (confocal) and two-photon all-digital (photon counting) laser scanning microscope. The confocal component uses two avalanche photodiodes (APDs) as the fluorescence detector to achieve high sensitivity and to overcome the limited photon counting rate of a single APD (∼5 MHz).
W G, Wier +3 more
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Spherical-coordinate scanning confocal laser microscope
Optical Engineering, 1995We present the design, construction, and characterization of a spherical-coordinate scanning, reflection-mode confocal laser microscope (SCSLM). Two system designs are presented, namely a conventional bulk optics object-scanning design and a preliminary optical-fiber-based two-axis optics/one-axis object scanning version.
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Ultrastructural and confocal laser scanning microscopic examination of TUNEL‐positive cells
The Journal of Pathology, 1997TdT-mediated dUTP-biotin nick end labelling (TUNEL) has been widely used for detecting cells with DNA fragmentation or apoptotic cells. However, since the concept of apoptosis is based on cellular ultrastructure, it is important to identify the morphological features of TUNEL-positive cells.
K, Kimura +9 more
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Optically Corrected Diode Laser for Confocal Scanning Microscope
Frontiers in Optics 2007/Laser Science XXIII/Organic Materials and Devices for Displays and Energy Conversion, 2007We propose the use of optically corrected laser diode in the confocal scanning microscope. The resulted beam has no astigmatism and produces a round diffraction limited spot on a sample.
Dmitry Rozhetskin +2 more
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The Intermediate Optical System of Laser-scanning Confocal Microscopes
1990This text explains some of the basics of intermediate optical systems in confocal microscopes. Important microscope components are discussed in more detail in other chapters: lasers (Chapter 5) and other light sources (Chapter 6), objective lenses (Chapter 7), special optical elements (Chapter 3), fibers (Chapter 26), and detectors (Chapter 12 ...
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A video rate confocal laser scanning microscope
Optical Society of America Annual Meeting, 1992A video rate confocal laser scanning microscope (CLSM) for biological applications has been developed for manufacture. This microscope is used for viewing thin "optical sections" of a sample by rejecting the signal from out-of-focus planes. The microscope consists of a multiline argon ion laser, a galvanometer and acousto-optic deflector (AOD) for ...
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Scanning and detection techniques used in a confocal scanning laser microscope
Journal of Microscopy, 1990SUMMARYA two‐mirror scanning mechanism for confocal microscopy is described. No optical components, in addition to the scanning mirrors, are used. Design criteria and performance of the scanner are discussed. The photometric linearity of a detector unit incorporating a photomultiplier tube is reported, and a dual detector unit with tunable split ...
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Automatic analysis for neuron by confocal laser scanning microscope
SPIE Proceedings, 2005The aim of this study is to develop a system that recognizes both the macro- and microscopic configurations of nerve cells and automatically performs the necessary 3-D measurements and functional classification of spines. The acquisition of 3-D images of cranial nerves has been enabled by the use of a confocal laser scanning microscope, although the ...
Kouhei Satou +4 more
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GFP fluorescence imaging with laser confocal scanning microscope
SPIE Proceedings, 1999ABSTRACT With gene marking technique, green fluorescent protein (GFP) and nodule bacteria gene has been linked together toform a single fusion gene expression vector. Then the vector is transferred into the nodule bacteria and the astragalus sinicusis invaded by the vector.
Yanhua Yu +3 more
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Confocal laser scanning microscope in the cell biology investigations
Proceedings of CAOL 2005. Second International Conference on Advanced Optoelectronics and Lasers, 2005., 2006A confocal microscope is a modern tool of cell biology that allows carrying out the investigations of different aspects of plant cell behavior both in the control and under the influence of external agents on the high scientific level. The confocal laser microscope with the appropriate software is designed for studying intracellular calcium ...
M.A. Sobol, V.A. Yatsenko
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