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Compact laser scanning confocal microscope
SPIE Proceedings, 2005Within the framework of the General Support Technology Program of the European Space Agency (ESA), a compact dedicated confocal laser scanning microscope has been developed for 3D fluorescence imaging of biological samples. The microscope permits normal confocal mode operation with excitation at 488nm and fluorescence lifetime imaging (FLIM) with ...
D. Beghuin +4 more
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Measurements Validating the Confocal Scanning Laser Holography Microscope
Microscopy and Microanalysis, 2011AbstractA confocal scanning laser holography (CSLH) microscope that uniquely combines the concepts of confocal microscopy with holography has been validated for making nonintrusive, full three-dimensional (3D) intensity and phase measurements of objects from a single viewpoint of observation without loss of object information.
Peter B, Jacquemin, Rodney A, Herring
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System integration for real-time laser scanning confocal microscope
2016 24th Signal Processing and Communication Application Conference (SIU), 2016In this work, a laser scanning confocal microscopy system governed by a software controlled DAQ Card is presented. The presented system can be utilized for scanning a target and displaying the resulting image through a designed graphical user interface (GUI).
Gokhan Gumus +2 more
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Novel Micro Scanning with Integrated Atomic Force Microscope and Confocal Laser Scanning Microscope
2019 IEEE Conference on Control Technology and Applications (CCTA), 2019Integrated atomic force microscope (AFM) and confocal laser scanning microscope (CLSM) can quickly obtain the three-dimensional (3-D) surface of the sample in large scanning range and recover the region of interesting (ROI) in nanoscale resolution. However, the traditional cooperative algorithm for integrated microscopes occupies too much scanning time.
Meng-Hao Chou +4 more
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A video rate laser scanning confocal microscope
SPIE Proceedings, 2008A video-rate laser scanning microscope was developed as an imaging engine to integrate with other photonic building blocks to fulfill various microscopic imaging applications. The system is quipped with diode laser source, resonant scanner, galvo scanner, control electronic and computer loaded with data acquisition boards and imaging software. Based
Hongzhou Ma +3 more
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Variable-ambient scanning stage for a laser scanning confocal microscope
Review of Scientific Instruments, 2003A variable-ambient scanning stage for a laser scanning confocal microscope was designed and tested. The stage allows for facile observation of both thin films and single molecule samples under inert conditions. High precision images have been acquired up to 174 μm2 using a single piezoceramic tube.
D. J. Sirbuly +4 more
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Identification of Surface Topography Scanned by Laser Scanning Confocal Microscope
Applied Mechanics and Materials, 2014Topography and surface roughness is defined as a set of surface textures formed due to used machining or other production technology. It is a key factor mainly for dynamically stressed components. Greater surface roughness negatively affects the fatigue strength of component or its wear resistance.The paper deals with the visualization and processing ...
Tomas Bezak +5 more
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A Confocal Laser Scanning Microscope Investigation of the Epiphany Obturation System
Journal of Endodontics, 2007The purpose of this study was to assess the sealer-dentin interface and compare the percentage and average depth of dentinal tubule sealer penetration in the coronal, middle and apical thirds of teeth obturated with the Epiphany Obturation System using 10x and 40x confocal laser scanning microscopy.
Saman R, Gharib +4 more
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Three-dimensional microscopy using a confocal laser scanning microscope
Optics Letters, 1985In a scanning laser microscope detecting fluorescent light from the specimen, the depth-discriminating property of confocal scanning has been used to carry out optical slicing of a thick specimen. The recorded digital images constitute a three-dimensional raster covering a volume of the specimen.
Kjell Carlsson, Reiner Lenz
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A UV laser-scanning confocal microscope for the measurement of intracellular Ca2+
Cell Calcium, 1994Modifications to the optics of a conventional confocal laser-scanning microscope were made to allow imaging intracellular Ca(2+)-dependent fluorescence with a UV laser (351 or 364 nm). Modifications included: (1) a chromatic compensation lens in the laser path; (2) the design of a practically achromatic relay lens; (3) a longer tube length for the ...
K, Kuba, S Y, Hua, T, Hayashi
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