Results 71 to 80 of about 3,145,730 (353)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

A compact, continuous-wave terahertz source based on a quantum-cascade laser and a miniature cryocooler

, 2010
We report on the development of a compact, easy-to-use terahertz radiation source, which combines a quantum cascade laser (QCL) operating at 3.1 THz with a compact, low input-power Stirling cooler.
Semenov, Alexey, Richter, Heiko, Schrottke, Lutz, Grahn, H. T., Giehler, M., Wienold, Martin, Huebers, H.-W., Pavlov, Sergey, Greiner-Bär, Michael, Hey, R., Semenov, Alexei D.   +10 more
core   +1 more source

Dual‐Comb Interferometry for Coherence Analysis of Tightly Locked Mid‐Infrared Quantum Cascade Laser Frequency Combs

Advanced Photonics Research
Frequency combs are powerful tools for many applications and high performances are achieved by stabilizing these lasers. For operation in the mid‐infrared, quantum cascade lasers (QCL) are ideal candidates as they present numerous advantages.
Alexandre Parriaux, Kenichi N. Komagata, Mathieu Bertrand, Valentin J. Wittwer, Jérôme Faist, Thomas Südmeyer   +5 more
doaj   +1 more source

Simulation of the temperature drift of the laser gyroscope path length

RUDN Journal of Engineering Research, 2023
The authors present the results of modeling the temperature drift of the resonator path length of a laser gyroscope based on a ring helium-neon laser with circular polarization of radiation and a magneto-optical frequency bias based on the Zeeman effect ...
Yaroslav A. Zubarev, Anton O. Sinelnikov, Victoria U. Mnatsakanyan   +2 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Laser frequency stabilization using a spatially multiplexed microfabricated Rb vapor cell

Applied Physics Express
We demonstrate a laser stabilization based on a spatially-multiplexed, microfabricated rubidium vapor cell. This design integrates multiple optical apertures within a cell, enabling a single input beam to simultaneously capture both Doppler-broadened and
Seji Kang, Soyeon Choi, Jaeuk Baek, Sang Eon Park, Jongcheol Park, Yeeun Na, Taeg Yong Kwon, Sang-Bum Lee, Jae Hoon Lee, Sangwon Seo, Young-Ho Park, Meung Ho Seo, Hyun-Gue Hong   +12 more
doaj   +1 more source

Extreme nonlinear response of ultranarrow optical transitions in cavity QED for laser stabilization [PDF]

, 2011
Weexplorethepotentialofdirectspectroscopyofultranarrowopticaltransitionsofatomslocalizedinanoptical cavity. In contrast to stabilization against a reference cavity, which is the approach currently used for the most highly stabilized lasers, stabilization
Michael J. Martin, D. Meiser, J. Thomsen, Jun Ye, M. Holland   +4 more
semanticscholar   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source