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Biophysical Studies of LC3 Family Proteins
2019Autophagy is an important cellular process in which cell components are degraded in a controlled way and their building blocks are recycled into new macromolecules. Autophagy starts within a double-membrane container, the autophagosome, itself the result of a number of interconversions of cell membranous elements.
Hervás, Javier H. +2 more
openaire +3 more sources
Semisynthetic Lipidated LC3 Protein Mediates Membrane Fusion
ChemBioChem, 2013All together: Lipidated LC3 has been synthesized by expressed protein ligation. A TEV-cleavable MBP tag was employed to facilitate ligation under folding conditions and to solubilize the lipidated protein. The synthetic LC3-phosphatidylethanolamine (PE) mediates membrane tethering and fusion at the physiological concentration of PE, and could be a ...
Yang, A. +4 more
openaire +3 more sources
Cement and Concrete Research, 2019
This paper presents data on the chloride diffusion coefficient (Dcl), ageing coefficient (m) and chloride threshold (Clth) related to seven concrete mixes (four M35 and three M50) with OPC, OPC + PFA (pulverised fuel ash) and limestone-calcined clay ...
R. Pillai +6 more
semanticscholar +1 more source
This paper presents data on the chloride diffusion coefficient (Dcl), ageing coefficient (m) and chloride threshold (Clth) related to seven concrete mixes (four M35 and three M50) with OPC, OPC + PFA (pulverised fuel ash) and limestone-calcined clay ...
R. Pillai +6 more
semanticscholar +1 more source
Investigation of C-A-S-H composition, morphology and density in Limestone Calcined Clay Cement (LC3)
Cement and Concrete Research, 2019In this study, the C-A-S-H composition, morphology and density are characterized for Limestone Calcined Clay Cement (LC3-50) blends containing clays with various kaolinite contents.
F. Avet +2 more
semanticscholar +1 more source
Quantification of autophagy flux using LC3 ELISA
Analytical Biochemistry, 2017Macroautophagy (hereafter referred to as autophagy) is a degradation system that delivers cytoplasmic materials to lysosomes via autophagosomes. Autophagic flux is defined as a measure of autophagic degradation activity. Despite several methods for monitoring autophagic flux being currently utilized, interest in finding a highly accurate, sensitive and
Sung-hee Oh +4 more
openaire +2 more sources
LC3/GABARAP family proteins: autophagy‐(un)related functions
The FASEB Journal, 2016ABSTRACT From yeast to mammals, autophagy is an important mechanism for sustaining cellular homeostasis through facilitating the degradation and recycling of aged and cytotoxic components. During autophagy, cargo is captured in double‐membraned vesicles, the autophagosomes, and degraded through lysosomal fusion ...
Schaaf, Marco B. E. +3 more
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LC3-I conversion to LC3-II does not necessarily result in complete autophagy.
International journal of molecular medicine, 2009Autophagy was induced in human neuroblastoma SH-SY5Y cells by two different procedures: deprivation of fetal serum in culture medium, or treatment with dopamine. 3-methyladenine prevented autophagy in the two procedures. Although it is usually considered that the conversion of soluble LC3-I to lipid bound LC3-II is associated with the formation of ...
Pol, Giménez-Xavier +4 more
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Autogenous and total shrinkage of limestone calcined clay cement (LC3) concretes
Construction and Building Materials, 2022Quang Dieu Nguyen +5 more
semanticscholar +1 more source