Results 51 to 60 of about 3,350,276 (277)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Non-linear unsteady aerodynamic model for insect-like flapping wings in the hover. Part 1: Methodology and analysis [PDF]

, 2006
The essence of this two-part paper is the analytical, aerodynamic modelling of insect-like flapping wings in the hover for microair vehicle applications.
Knowles, Kevin, Zbikowski, Rafal, Ansari, S. A.   +2 more
core   +1 more source

Development of Delta Wing Aerodynamics Research in Universiti Teknologi Malaysia Low Speed Wind Tunnel

Advances in Mechanical Engineering, 2014
This paper presents wind tunnel experiment on two delta wing configurations which are differentiated by their leading edge profiles: sharp and round-edged wings. The experiments were performed as a part of the delta wing aerodynamics research development
Shabudin Mat, I. S. Ishak, Tholudin Mat Lazim, Shuhaimi Mansor, Mazuriah Said, Abdul Basid Abdul Rahman, Ahmad Shukeri Mohd. Kamaludim, Romain Brossay   +7 more
doaj   +1 more source

Continuous Reynolds number effect analysis of the influence of vortex generator on the aerodynamic performance of airfoil [PDF]

Journal of Hebei University of Science and Technology
In order to effectively improve the power generation efficiency of large wind turbines, studies on the optimization measures for the aerodynamic performance of wind turbine airfoils were conducted. Wind tunnel experiments were conducted to systematically
Yaya JIA, Jiacheng ZHAO, Wanpeng CAO, Qingkuan LIU, Shanning LYU   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Examination and Prediction of the Lift Components of Low Aspect Ratio Rectangular Flat Plate Wings

Aerospace, 2023
An investigation of the lift components present over low aspect ratio rectangular wings is presented. Wing aspect ratios ranging from 0.5 to 3 are examined using published experimental results and analytic analysis methods.
Lance W. Traub
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Leading Edge Vortex Development on a Pitch-Up Airfoil

Volume 1B, Symposia: Fluid Machinery; Fluid Power; Fluid-Structure Interaction and Flow-Induced Noise in Industrial Applications; Flow Applications in Aerospace; Flow Manipulation and Active Control: Theory, Experiments and Implementation; Fundamental Issues and Perspectives in Fluid Mechanics, 2013
The dynamics of leading edge vortex (LEV) on an airfoil due to pitch-up motion is investigated using computational fluid dynamics techniques to solve the Navier-Stokes equations on composite overlapping grids. The objectives are to (1) quantify the contribution of circulatory effects caused by vortex development, and non-circulatory effects due to ...
Kyle Hord, Yongsheng Lian
openaire   +1 more source

Enhancing Energy Harvesting Efficiency of Flapping Wings with Leading-Edge Magnus Effect Cylinder

Biomimetics
According to the Magnus principle, a rotating cylinder experiences a lateral force perpendicular to the incoming flow direction. This phenomenon can be harnessed to boost the lift of an airfoil by positioning a rotating cylinder at the leading edge.
Huaqiang Zhang, Bing Zhu, Weidong Chen
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source