Results 131 to 140 of about 3,640,039 (291)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Reducing variability of a valuable construction input : subcontractors
, 2015 Subcontractors are critical to the U.S. construction industry. They are used by general contractors (GC) to perform a majority of work. Subcontractors safely install components specified, in the time needed, and at a competitive cost.
Stevens, Matt (R19432)
core
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
BuildingsCurrent construction site conditions and practices often lead to various forms of waste, which in turn decreases productivity and value generation. Lean principles aim to minimize waste while maximizing value.
Karl Keyrouz +2 more
doaj +1 more source
Impacts of Lean Construction on Safety Systems: A System Dynamics Approach. [PDF]
Int J Environ Res Public Health, 2019 Wu X, Yuan H, Wang G, Li S, Wu G.
europepmc +1 more source
Lean construction as a strategic option : testing its suitability and acceptibility in Sri Lanka
, 2008 Lean Construction is a concept still new to many construction industries in the world. According to Ballard and Howell (2003) countries such as UK, Australia, USA and Brazil have gained significant benefits by adoption of Lean Construction concepts ...
Wijesiri, Duleesha +1 more
core
The application of a pilot pull planning system to construction projects [PDF]
, 2002 A new planning system was introduced as a pilot within a large UK construction company. The system, which attempts to address some of the problems of construction “front end” planning, is investigated relative to Lean Construction and specifically the ...
Johansen, Eric
core
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
BuildingsDigital transformation, increasing project complexity, and sustainability requirements are driving the adoption of advanced construction management approaches.
Mohammad Haroon Aman, Yachen Liu
doaj +1 more source
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source