Results 141 to 150 of about 15,302 (309)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Investigating the application of one piece flow from lean manufacturing in the construction delivery of mass housing projects. [PDF]

Sci Rep
Saad DA, Habib M, Abou-Zeid A.
europepmc   +1 more source

Lean Construction Techniques and Individual Performance [PDF]

, 2019
Shuquan Li, Meng Chao Fan, Xiuyu Wu
openalex   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Lean-NET-Based Local Brain Connectome Analysis for Autism Spectrum Disorder Classification. [PDF]

Bioengineering (Basel)
Chelef A, Yuksel Dal D, Ozturk M, Yousif MAA, Koc G.   +4 more
europepmc   +1 more source

Lean Construction Supply Chain: A Bibliometric Analysis of the Knowledge Base

, 2019
Temidayo Oluwasola Osunsanmi, Ayodeji Emmanuel Oke, Clinton Aigbavboa   +2 more
openalex   +2 more sources

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Evaluation of ten diagnostic models for metabolic dysfunction-associated fatty liver disease in lean people living with HIV. [PDF]

BMC Infect Dis
Xu W, Liu D, Zhang R, Chen J, Shen Y.
europepmc   +1 more source

Déploiement de la théorie du Lean dans la chaîne de valeur de la construction

, 2016
Zakaria Dakhli
openalex   +1 more source