Results 91 to 100 of about 411,689 (282)

Comparison of lentiviral vector titration methods

BMC Biotechnology, 2006
Background Lentiviral vectors are efficient vehicles for stable gene transfer in dividing and non-dividing cells. Several improvements in vector design to increase biosafety and transgene expression, have led to the approval of these vectors for use in ...
Debyser Zeger, Baekelandt Veerle, Willems Sofie, Geraerts Martine, Gijsbers Rik   +4 more
doaj   +1 more source

Lentiviral Vectors to Study Stochastic Noise in Gene Expression

, 2011
Lentiviral vectors are vehicles for gene delivery that were originally derived from the human immunodeficiency virus type-1 (HIV-1) lentivirus. These vectors are defective for replication, and thus considered relatively safe, but are capable of stably ...
Franz, Kate, Weinberger, Leor S., Kate Franz, Singh, Abhyudai, Abhyudai Singh, Leor S. Weinberger   +5 more
core   +1 more source

Single‐Cell Dissection of Microglial Dysfunction in Diabetes‐Associated Neurodegenerative Vulnerability

Advanced Science, EarlyView.
Using single‐cell transcriptomic and epigenomic profiling, researchers mapped microglial dysfunction across the lifespan of db/db mice. The authors uncovered a nonlinear microglial remodeling featuring early inflammatory priming, a mid‐stage transcriptional inflection, and late dysfunction.
Jizhou Zhang, Tsz Kwan Yau, Joyce Tse, Tsz Ho Li, Dongjie Hu, Yong Juan Zhao, Ting‐Fung Chan   +6 more
wiley   +1 more source

Genetic alteration of SJ293TS cells and modification of serum-free media enhances lentiviral vector production

Molecular Therapy: Methods & Clinical Development
Successful cell and gene therapy clinical trials have resulted in the US Food and Drug Administration and European Medicines Agency approving their use for treatment of patients with certain types of cancers and monogenetic diseases.
Matthew Bauler, Francesca Ferrara, Brandon Lowe, Jordan A. Beard, Chris Wincek, Matthew M. Wielgosz, Jeoungeun J. Park, Na Shang, Saikat Nandy, Cai Li, Deanna M. Langfitt, Sheng Zhou, Robert E. Throm   +12 more
doaj   +1 more source

USP30‐Mediated Deubiquitination of PEX5 Suppresses Pexophagy to Drive Tubular Injury in Diabetic Kidney Disease

Advanced Science, EarlyView.
USP30 accumulation in diabetic renal tubular epithelial cells deubiquitinates PEX5, suppresses pexophagy, and drives dysfunctional peroxisome accumulation and tubular injury. USP30 depletion restores PEX5 ubiquitination and pexophagy, improves peroxisomal function, and alleviates tubular damage, highlighting USP30 as a therapeutic target in diabetic ...
Jia Li, Chaoyang Hua, Guangpu Li, Furong Zhai, Jingjing Ren, Shaokang Pan, Qi Feng, Jianguo Wen, Dongwei Liu, Zhangsuo Liu, Jiayu Duan   +10 more
wiley   +1 more source

Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors

Frontiers in Cellular Neuroscience, 2013
Astrocytes are now considered as key players in brain information processing because of their newly discovered roles in synapse formation and plasticity, energy metabolism and blood flow regulation.
Nicolas eMerienne, Juliette eLe Douce, Emilie eFaivre, Nicole eDéglon, Gilles eBonvento   +4 more
doaj   +1 more source

Novel fusion protein-expressing lentiviral vectors ameliorate collagen induced arthritis [PDF]

, 2010
Collagen induced arthritis (CIA) is a mouse model of autoimmunity that closely resembles human rheumatoid arthritis (RA), a debilitating disease with no cure.
Ward, E.M.
core  

Lentiviral vectors mediate efficient and stable gene transfer in adult neural stem cells in vivo [PDF]

, 2006
Modulation of adult neurogenesis may offer new therapeutic strategies for various brain disorders. In the adult mammalian brain the subventricular zone (SVZ) of the lateral ventricle is a region of continuous neurogenesis.
Eggermont, Kristel, Geraerts, Martine, Garcia-Verdugo, Jose-Manuel, Debyser, Zeger, Baekelandt, Veerle, Hernandez-Acosta, Pilar   +5 more
core   +1 more source

IFI27 and BAX are Essential for GSDME‐Mediated Myeloma Cell Pyroptosis

Advanced Science, EarlyView.
The proposed model of GSDME‐mediated MM cell pyroptosis modulated by IFI27 and BAX. IFI27 is downregulated in MM cells. Upon pyroptotic stimulation, such as DOX or ETO treatment, IFI27 is induced, therefore liberating BAX from BCL‐2 ❶. BAX is then recruited to mitochondria and forms homo‐oligomeric channels in OMM, therefore leading to cytochrome C ...
Yaoli Cui, Yuening Sun, Ziyang Liu, Xuntao Lai, Yunshu Zhao, Yan'er Wang, Yuanming He, Fang Xu, Du Feng, Longlong Liu, Zhenqian Huang, Lijie Xing, Xinliang Mao   +12 more
wiley   +1 more source

The CXCL5/CXCR2 Axis Attenuates Ferroptosis in Intrahepatic Cholangiocarcinoma Through the Positive Feedback Loop Between PTGS2 Transcriptional Activation and Neutrophil Recruitment

Advanced Science, EarlyView.
The CXCL5/CXCR2 axis directly inhibits ferroptosis in ICC cells by activating the NF‐κB/PTGS2 pathway. Meanwhile, the CXCL5/CXCR2 axis recruits N2 TANs, and the activated PTGS2 promotes the secretion of TNF‐α from the recruited N2 TANs via the CCL2/CCL7/CCR2 pathway, thereby activating the NF‐κB/PTGS2 axis and forming a positive feedback loop, which ...
Chanqi Ye, Kefeng Lin, Shuyao Yang, Yan Zhang, Wenli Gao, Jiaqi Chen, Ying Dong, Rui Bai, Yang Tian, Minghao Wang, Zhijun Yuan, Yuxiao Ma, Yuqin He, Ruyin Chen, Wenguang Fu, Qi Jiang, Qiong Li, Jian Ruan   +17 more
wiley   +1 more source