Results 1 to 10 of about 157,834 (170)

Primate and Feline Lentivirus Vector RNA Packaging and Propagation by Heterologous Lentivirus Virions [PDF]

Journal of Virology, 2001
ABSTRACT Development of safe and effective gene transfer systems is critical to the success of gene therapy protocols for human diseases. Currently, several primate lentivirus-based gene transfer systems, such as those based on human and simian immunodeficiency viruses (HIV/SIV), are being tested; however, their use in humans raises safety ...
M T, Browning, R D, Schmidt, K A, Lew, T A, Rizvi   +3 more
openaire   +2 more sources

A Distinct Subset of Highly Proliferative and Lentiviral Vector (LV)-Transducible NK Cells Define a Readily Engineered Subset for Adoptive Cellular Therapy

Frontiers in Immunology, 2019
Genetic engineering is an important tool for redirecting the function of various types of immune cells and their use for therapeutic purpose. Although NK cells have many beneficial therapeutic features, genetic engineering of immune cells for targeted ...
Rafijul Bari, Markus Granzin, Kam Sze Tsang, Andre Roy, Winfried Krueger, Rimas Orentas, Dina Schneider, Rita Pfeifer, Nina Moeker, Els Verhoeyen, Els Verhoeyen, Boro Dropulic, Wing Leung   +12 more
doaj   +1 more source

Identifying the Conditions Under Which Antibodies Protect Against Infection by Equine Infectious Anemia Virus

Vaccines, 2014
The ability to predict the conditions under which antibodies protect against viral infection would transform our approach to vaccine development. A more complete understanding is needed of antibody protection against lentivirus infection, as well as the ...
Elissa J. Schwartz, Robert J. Smith?
doaj   +1 more source

Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography

Veterinární Medicína, 2001
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal binding domain in pRSET-B vector.
D. Molinková
doaj   +1 more source

Sources of Error in Mammalian Genetic Screens

G3: Genes, Genomes, Genetics, 2016
Genetic screens are invaluable tools for dissection of biological phenomena. Optimization of such screens to enhance discovery of candidate genes and minimize false positives is thus a critical aim.
Laura Magill Sack, Teresa Davoli, Qikai Xu, Mamie Z. Li, Stephen J. Elledge   +4 more
doaj   +1 more source

Impact of Veterinary Vaccines on Human Health and Probable Challenges

Acta Veterinaria Eurasia, 2021
Md. Mehadi Hasan SOHAG, Yahia CHEBLOUNE   +1 more
doaj   +1 more source

Specific Inhibition of SRC Kinase Impairs Malignant Glioma Growth In Vitro and In Vivo

Molecular Therapy: Nucleic Acids, 2012
Malignant glioma is a severe cancer with a poor prognosis. Local occurrence and rare metastases of malignant glioma make it a suitable target for gene therapy. Several studies have demonstrated the importance of Src kinase in different cancers.
Hanna Stedt, Laura Alasaarela, Haritha Samaranayake, Jere Pikkarainen, Ann-Marie Määttä, Ivana Kholová, Aaron S. Parker, Seppo Ylä-Herttuala   +7 more
doaj   +1 more source

Development of Viral Vectors for Use in Cardiovascular Gene Therapy

Viruses, 2010
Cardiovascular disease represents the most common cause of mortality in the developed world but, despite two decades of promising pre-clinical research and numerous clinical trials, cardiovascular gene transfer has so far failed to demonstrate convincing
Paul A. Kingston, Salik J. Kakar, Parisa Ranjzad, Paul D. Williams   +3 more
doaj   +1 more source

An Efficient Method for Isolation of Plasmid DNA for Transfection of Mammalian Cell Cultures

Methods and Protocols, 2020
In this article, we present several protocols that describe the steps from cloning and obtaining a large amount of pure plasmid DNA to generation of lentiviruses based on these constructs.
Daniel V. Kachkin, Julia I. Khorolskaya, Julia S. Ivanova, Aleksandr A. Rubel   +3 more
doaj   +1 more source

Global prevalence and risk factors of equine infectious anemia: A systematic review and meta-analysis [PDF]

Veterinary World
Background and Aim: Equine infectious anemia (EIA) is a lentiviral disease affecting members of the Equidae family, with global distribution and significant implications for animal health and biosecurity.
Lintang Winantya Firdausy, Faisal Fikri, Arya Pradana Wicaksono, Hakan Çalışkan, Muhammad Thohawi Elziyad Purnama   +4 more
doaj   +1 more source