Results 41 to 50 of about 166,720 (266)
Finding novel vulnerabilities of hypomorphic BRCA1 alleles
Molecular Oncology, EarlyView.Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder +10 more
wiley +1 more source
Cultured adipocytes transfected with lentivirus.
, 2019 (a) Control (CL-Dox-), (b) Control with lentivirus switched on (CL-Dox+), (c) CL treated (CL+ Dox-), (d) CL treated adipocytes with lentivirus switched on (CL+Dox+).
Nicole Wen Mun Khor (7907894) +2 more
core +1 more source
Foamy Virus Vectors for HIV Gene Therapy
Viruses, 2013 Highly active antiretroviral therapy (HAART) has vastly improved outcomes for patients infected with HIV, yet it is a lifelong regimen that is expensive and has significant side effects.
Grant D. Trobridge, Miles E. Olszko
doaj +1 more source
MITF maintains genome stability in nonmelanocyte lineages
Molecular Oncology, EarlyView.MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir +13 more
wiley +1 more source
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Lentivirus-mediated NIBP knockdown in HCT116 cells.
, 2017 Control un-transfected HCT116 cells had higher NIBP protein expression compared to NIBP-knockdown HCT116 cells. Cells transfected with an empty vector (NC) had high NIBP expression, similar to the control un-transfected HCT116 cells, as determined by ...
Mengbin Qin (3710482) +6 more
core +1 more source
MiR‐513a promotes human erythroid differentiation by modulating c‐Jun
FEBS Open Bio, EarlyView.During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim +11 more
wiley +1 more source
, 2023 The relative contribution of somatically point mutation variants between DMKN-shRNA lentivirus (KD) and GFP lentivirus (NC) control in MUM-2B cell line.
Saber Imani (6680012) +9 more
core +1 more source
Engineering a DYRK1B R102C mutation: insights into metabolic syndrome pathogenesis through lentiviral gene delivery [PDF]
Medical Laboratory JournalBackground: A rare heterozygous DYRK1B mutation (R102C) recently linked to a familial form of metabolic syndrome prompted this study to introduce the R102C mutation into the mouse DYRK1B gene, utilizing recombinant lentiviruses for long-term gene ...
Afrooz Daneshparvar +6 more
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