Results 41 to 50 of about 173,548 (215)
Exploration of high-efficiency transfection methods for sheep fibroblasts OAR-L1
浙江大学学报. 农业与生命科学版, 2022 In order to achieve high-efficiency expression of exogenous protein in sheep lung fibroblasts OAR-L1, and to explore a suitable transfection method for the cell line, the transfection efficiencies of polyethyleneimine (PEI), LipofectamineTM 2000 ...
WU Fei +9 more
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Gene therapy for the neurological manifestations in lysosomal storage disorders
Journal of Lipid Research, 2014 Over the past several years, considerable progress has been made in the development of gene therapy as a therapeutic strategy for a variety of inherited metabolic diseases, including neuropathic lysosomal storage disorders (LSDs).
Seng H. Cheng
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eLife, 2016 Viruses manipulate host factors to enhance their replication and evade cellular restriction. We used multiplex tandem mass tag (TMT)-based whole cell proteomics to perform a comprehensive time course analysis of >6500 viral and cellular proteins ...
Edward JD Greenwood +6 more
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Intramuscular Delivery of Gene Therapy for Targeting the Nervous System
Frontiers in Molecular Neuroscience, 2020 Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease.
Andrew P. Tosolini +2 more
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Common Modular Structure of Lentivirus LTRs [PDF]
, 1996 Retroviruses are expressed under the control of viral control regions designated long terminal repeats (LTRs), which contain all signals for transcriptional initiation as well as transcriptional termination.
Frech, Kornelie +2 more
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FIV: from lentivirus to lentivector [PDF]
The Journal of Gene Medicine, 2004 Molecular virological understanding of the feline immunodeficiency virus (FIV) life cycle is increasing, facilitating rational derivation of improved vectors from this non-primate lentivirus. The packaging signal has been mapped, a central DNA flap has been identified, and class I integrase mutants have been validated.
Dyana T, Saenz, Eric M, Poeschla
openaire +2 more sources
Molecular Studies of HTLV-1 Replication: An Update
Viruses, 2016 Human T-cell leukemia virus type 1 (HTLV-1) was the first human retrovirus discovered. Studies on HTLV-1 have been instrumental for our understanding of the molecular pathology of virus-induced cancers.
Jessica L. Martin +4 more
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Development and Optimization of a High Titer Recombinant Lentivirus System
, 2018 To enable simple and effective high titer recombinant lentivirus production, we examined key parameters for the generation of lentivirus including: transfection conditions, DNA vector selection, media change, and incubation time.
core +3 more sources
A Versatile Tool for Stable Inhibition of microRNA Activity
Biology, 2013 MicroRNAs (miRNAs) are a class of small RNAs (18–22 nt) that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing.
Ileana Zucchi +9 more
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Turkish Journal of Hematology, 2023 Objective: Hemophilia A is an X-linked recessive bleeding disorder caused by a deficiency of plasma coagulation factor VIII (FVIII), and it accounts for about 80%-85% of all cases of hemophilia.
Cansu Hemşinlioğlu +14 more
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