Results 21 to 30 of about 166,720 (266)
Beyond retrovirus infection: HIV meets gene therapy
Genetics and Molecular Biology, 2006 The human immunodeficiency virus (HIV) is classified as a retrovirus because of its RNA genome and the fact that it requires reverse transcriptase to convert it into DNA.
Flávia Helena da Silva +2 more
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Retrogradely Transportable Lentivirus Tracers for Mapping Spinal Cord Locomotor Circuits
Frontiers in Neural Circuits, 2018 Retrograde tracing is a key facet of neuroanatomical studies involving long distance projection neurons. Previous groups have utilized a variety of tools ranging from classical chemical tracers to newer methods employing viruses for gene delivery.
Imran S. Sheikh +7 more
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Engineering Novel Lentiviral Vectors for Labelling Tumour Cells and Oncogenic Proteins
Bioengineering, 2022 Lentiviral vectors are unique and highly efficient genetic tools to incorporate genetic materials into the genome of a variety of cells whilst conserving biosafety.
Seçkin Akgül +3 more
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Retroviral Integration Site Selection
Viruses, 2010 The stable insertion of a copy of their genome into the host cell genome is an essential step of the life cycle of retroviruses. The site of viral DNA integration, mediated by the viral-encoded integrase enzyme, has important consequences for both the ...
Angela Ciuffi, Sébastien Desfarges
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Molecular Therapy: Methods & Clinical Development, 2017 The success of lentiviral vectors in curing fatal genetic and acquired diseases has opened a new era in human gene therapy. However, variability in the efficacy and safety of this therapeutic approach has been reported in human patients.
Thipparat Suwanmanee +6 more
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Exploration of high-efficiency transfection methods for sheep fibroblasts OAR-L1
浙江大学学报. 农业与生命科学版, 2022 In order to achieve high-efficiency expression of exogenous protein in sheep lung fibroblasts OAR-L1, and to explore a suitable transfection method for the cell line, the transfection efficiencies of polyethyleneimine (PEI), LipofectamineTM 2000 ...
WU Fei +9 more
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Gene therapy for the neurological manifestations in lysosomal storage disorders
Journal of Lipid Research, 2014 Over the past several years, considerable progress has been made in the development of gene therapy as a therapeutic strategy for a variety of inherited metabolic diseases, including neuropathic lysosomal storage disorders (LSDs).
Seng H. Cheng
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Development and Optimization of a High Titer Recombinant Lentivirus System
, 2018 To enable simple and effective high titer recombinant lentivirus production, we examined key parameters for the generation of lentivirus including: transfection conditions, DNA vector selection, media change, and incubation time.
core +3 more sources
eLife, 2016 Viruses manipulate host factors to enhance their replication and evade cellular restriction. We used multiplex tandem mass tag (TMT)-based whole cell proteomics to perform a comprehensive time course analysis of >6500 viral and cellular proteins ...
Edward JD Greenwood +6 more
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Intramuscular Delivery of Gene Therapy for Targeting the Nervous System
Frontiers in Molecular Neuroscience, 2020 Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease.
Andrew P. Tosolini +2 more
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