Results 31 to 40 of about 173,548 (215)

Lentivirus-mediated circMEF2A1 function investigation in vivo.

, 2023
(A, B) qRT-PCR analysis of circMEF2A1 in the cDNA samples generated from the breast muscles of LV-si-circMEF2A1, LV-si-NC, LV-ov-circMEF2A1, and LV-ov-NC infected chicks, n = 3.
Yuqi Chen (602230), Xiyu Zhao (11510077), Haorong He (9608033), Qing Zhu (349093), Jing Zhao (21160), Yuanhang Wei (9608036), Yao Zhang (134381), Xiaoxu Shen (4020461), Yifeng Zhu (1519378), Shunshun Han (4020455), Huadong Yin (832156)   +10 more
core   +1 more source

INSPIIRED: A Pipeline for Quantitative Analysis of Sites of New DNA Integration in Cellular Genomes

Molecular Therapy: Methods & Clinical Development, 2017
Integration of new DNA into cellular genomes mediates replication of retroviruses and transposons; integration reactions have also been adapted for use in human gene therapy.
Eric Sherman, Christopher Nobles, Charles C. Berry, Emmanuelle Six, Yinghua Wu, Anatoly Dryga, Nirav Malani, Frances Male, Shantan Reddy, Aubrey Bailey, Kyle Bittinger, John K. Everett, Laure Caccavelli, Mary J. Drake, Paul Bates, Salima Hacein-Bey-Abina, Marina Cavazzana, Frederic D. Bushman   +17 more
doaj   +1 more source

Lentivirus-mediated knockdown of P27RF-Rho inhibits hepatocellular carcinoma cell growth [PDF]

, 2017
Aim of the study: To investigate the effects of P27RF-Rho on hepatocellular carcinoma (HCC) cell growth and explore the possibility of using it as a novel therapeutic target for liver cancer treatment.
Guofu Chen, Guoyue Lv, Shuli Xie, Mingguang Zhu, Guangyi Wang   +4 more
core   +1 more source

HIV Diagnosis and Treatment through Advanced Technologies

Frontiers in Public Health, 2017
Human immunodeficiency virus (HIV) is the chief contributor to global burden of disease. In 2010, HIV was the fifth leading cause of disability-adjusted life years in people of all ages and leading cause for people aged 30–44 years. It is classified as a
Hafiza Fizzah Zulfiqar, Aneeqa Javed, Sumbal, Bakht Afroze, Qurban Ali, Khadija Akbar, Tariq Nadeem, Muhammad Adeel Rana, Zaheer Ahmad Nazar, Idrees Ahmad Nasir, Tayyab Husnain   +10 more
doaj   +1 more source

Chemical modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons

eLife, 2019
Direct conversion of human somatic fibroblasts into induced neurons (iNs) allows for the generation of functional neurons while bypassing any stem cell intermediary stages.
Joseph Herdy, Simon Schafer, Yongsung Kim, Zoya Ansari, Dina Zangwill, Manching Ku, Apua Paquola, Hyungjun Lee, Jerome Mertens, Fred H Gage   +9 more
doaj   +1 more source

Beyond retrovirus infection: HIV meets gene therapy

Genetics and Molecular Biology, 2006
The human immunodeficiency virus (HIV) is classified as a retrovirus because of its RNA genome and the fact that it requires reverse transcriptase to convert it into DNA.
Flávia Helena da Silva, Tiago Pires Dalberto, Nance Beyer Nardi   +2 more
doaj   +1 more source

Retrogradely Transportable Lentivirus Tracers for Mapping Spinal Cord Locomotor Circuits

Frontiers in Neural Circuits, 2018
Retrograde tracing is a key facet of neuroanatomical studies involving long distance projection neurons. Previous groups have utilized a variety of tools ranging from classical chemical tracers to newer methods employing viruses for gene delivery.
Imran S. Sheikh, Kathleen M. Keefe, Noelle A. Sterling, Ian P. Junker, Chidubem I. Eneanya, Yingpeng Liu, Xiao-Qing Tang, George M. Smith   +7 more
doaj   +1 more source

Engineering Novel Lentiviral Vectors for Labelling Tumour Cells and Oncogenic Proteins

Bioengineering, 2022
Lentiviral vectors are unique and highly efficient genetic tools to incorporate genetic materials into the genome of a variety of cells whilst conserving biosafety.
Seçkin Akgül, Carolin Offenhäuser, Anja Kordowski, Bryan W. Day   +3 more
doaj   +1 more source

Retroviral Integration Site Selection

Viruses, 2010
The stable insertion of a copy of their genome into the host cell genome is an essential step of the life cycle of retroviruses. The site of viral DNA integration, mediated by the viral-encoded integrase enzyme, has important consequences for both the ...
Angela Ciuffi, Sébastien Desfarges
doaj   +1 more source

Toward Personalized Gene Therapy: Characterizing the Host Genetic Control of Lentiviral-Vector-Mediated Hepatic Gene Delivery

Molecular Therapy: Methods & Clinical Development, 2017
The success of lentiviral vectors in curing fatal genetic and acquired diseases has opened a new era in human gene therapy. However, variability in the efficacy and safety of this therapeutic approach has been reported in human patients.
Thipparat Suwanmanee, Martin T. Ferris, Peirong Hu, Tong Gui, Stephanie A. Montgomery, Fernando Pardo-Manuel de Villena, Tal Kafri   +6 more
doaj   +1 more source