Results 31 to 40 of about 157,834 (170)
Engineering Novel Lentiviral Vectors for Labelling Tumour Cells and Oncogenic Proteins
Bioengineering, 2022 Lentiviral vectors are unique and highly efficient genetic tools to incorporate genetic materials into the genome of a variety of cells whilst conserving biosafety.
Seçkin Akgül +3 more
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Retroviral Integration Site Selection
Viruses, 2010 The stable insertion of a copy of their genome into the host cell genome is an essential step of the life cycle of retroviruses. The site of viral DNA integration, mediated by the viral-encoded integrase enzyme, has important consequences for both the ...
Angela Ciuffi, Sébastien Desfarges
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Molecular Therapy: Methods & Clinical Development, 2017 The success of lentiviral vectors in curing fatal genetic and acquired diseases has opened a new era in human gene therapy. However, variability in the efficacy and safety of this therapeutic approach has been reported in human patients.
Thipparat Suwanmanee +6 more
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Exploration of high-efficiency transfection methods for sheep fibroblasts OAR-L1
浙江大学学报. 农业与生命科学版, 2022 In order to achieve high-efficiency expression of exogenous protein in sheep lung fibroblasts OAR-L1, and to explore a suitable transfection method for the cell line, the transfection efficiencies of polyethyleneimine (PEI), LipofectamineTM 2000 ...
WU Fei +9 more
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Gene therapy for the neurological manifestations in lysosomal storage disorders
Journal of Lipid Research, 2014 Over the past several years, considerable progress has been made in the development of gene therapy as a therapeutic strategy for a variety of inherited metabolic diseases, including neuropathic lysosomal storage disorders (LSDs).
Seng H. Cheng
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An endogenous lentivirus in the germline of a rodent
Retrovirology, 2022 Abstract Lentiviruses (genus Lentivirus ) are complex retroviruses that infect a broad range of mammals, including humans. Unlike many other retrovirus genera, lentiviruses have only rarely been incorporated into the mammalian germline.
Roziah Kambol +2 more
openaire +3 more sources
eLife, 2016 Viruses manipulate host factors to enhance their replication and evade cellular restriction. We used multiplex tandem mass tag (TMT)-based whole cell proteomics to perform a comprehensive time course analysis of >6500 viral and cellular proteins ...
Edward JD Greenwood +6 more
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Intramuscular Delivery of Gene Therapy for Targeting the Nervous System
Frontiers in Molecular Neuroscience, 2020 Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease.
Andrew P. Tosolini +2 more
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Molecular Studies of HTLV-1 Replication: An Update
Viruses, 2016 Human T-cell leukemia virus type 1 (HTLV-1) was the first human retrovirus discovered. Studies on HTLV-1 have been instrumental for our understanding of the molecular pathology of virus-induced cancers.
Jessica L. Martin +4 more
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A Versatile Tool for Stable Inhibition of microRNA Activity
Biology, 2013 MicroRNAs (miRNAs) are a class of small RNAs (18–22 nt) that post transcriptionally regulate gene expression by binding to complementary sequences on target mRNAs, resulting in translational repression or target degradation and gene silencing.
Ileana Zucchi +9 more
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