Results 51 to 60 of about 166,720 (266)

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Plasmonic Enhancement of Fluorescence and Protein Dynamics in Living Mammalian Cells

Advanced Materials, EarlyView.
This study demonstrates plasmonic enhancement of the function of fluorescent voltage sensing proteins (genetically encoded voltage indicators, (GEVIs), QuasAr6) in live mammalian cells. Coupling to plasmonic nanoparticles does not just increase fluorescence, but influences the protein photocycle, creating a hybrid sensor with its response speed to ...
Marco Locarno, Qiangrui Dong, Marco Post, Xin Meng, Cristiano Glessi, Nynke Marije Hettema, Nidas Brandsma, Sebbe Blokhuizen, Alejandro Castañeda Garcia, Srividya Ganapathy, Thieme Schmidt, Lars van Roemburg, Bing Xu, Chun‐Ting Cho, Liedewij Laan, Miao‐Ping Chien, Daan Brinks   +16 more
wiley   +1 more source

Study shRNA lentivirus vectors.

, 2015
a NA—not availableStudy shRNA lentivirus vectors.
Bo Ding (210928), Fiona McPhee (414636), Michele Agler (685695), Nannan Zhou (324509), Mark Cockett (16503)   +4 more
core   +1 more source

A Distinct Subset of Highly Proliferative and Lentiviral Vector (LV)-Transducible NK Cells Define a Readily Engineered Subset for Adoptive Cellular Therapy

Frontiers in Immunology, 2019
Genetic engineering is an important tool for redirecting the function of various types of immune cells and their use for therapeutic purpose. Although NK cells have many beneficial therapeutic features, genetic engineering of immune cells for targeted ...
Rafijul Bari, Markus Granzin, Kam Sze Tsang, Andre Roy, Winfried Krueger, Rimas Orentas, Dina Schneider, Rita Pfeifer, Nina Moeker, Els Verhoeyen, Els Verhoeyen, Boro Dropulic, Wing Leung   +12 more
doaj   +1 more source

GPR124 Alleviates Blood–Brain Barrier Disruption by Enhancing Microvascular Endothelial Function after Traumatic Brain Injury

Advanced Science, EarlyView.
Our study reveals the protective role of GPR124 in maintaining BBB integrity and promoting neurological recovery following TBI. It makes a significant contribution by uncovering a novel molecular interaction between GPR124 and FGFBP1 and linking this to activation of the Wnt/β‐catenin signaling pathway in vascular repair mechanisms.
Chen Wang, Lin Cai, Qiuyuan Gong, Yang Yang, Yuqing Liang, Xinyu Niu, Lai Wei, Ze Liu, Shengju Wu, Yinghui Men, Yaohui Tang, Jun Ding, Hengli Tian, Hao Chen   +13 more
wiley   +1 more source

shRNA lentivirus vector constructs used.

, 2014
shRNA lentivirus vector constructs used.
Claire Shannon-Lowe (166060), Martin Rowe (190734), Andrew D. Hislop (190727), Jianmin Zuo (190715), Rachel J. M. Abbott (619548), Rosemary J. Tierney (619549), Laura L. Quinn (619547)   +6 more
core   +1 more source

Identifying the Conditions Under Which Antibodies Protect Against Infection by Equine Infectious Anemia Virus

Vaccines, 2014
The ability to predict the conditions under which antibodies protect against viral infection would transform our approach to vaccine development. A more complete understanding is needed of antibody protection against lentivirus infection, as well as the ...
Elissa J. Schwartz, Robert J. Smith?
doaj   +1 more source

Malectin Alleviates Endoplasmic Reticulum Stress in Gestational Diabetes Mellitus via Glycoprotein Quality Control Mechanisms

Advanced Science, EarlyView.
Malectin alleviates high glucose‐induced ER stress and damage in placental trophoblasts, a function dependent on its six critical carbohydrate‐binding residues. In a GDM mouse model, administration of TAT‐Malectin ameliorated hyperglycemia and placental ER stress and prevented fetal macrosomia.
Jiahui Zhu, Yumeng Zhang, Ye Wang, Xiaoyu Zhu, Ailin Yuan, Weijing Yin, Huangmin Yu, Xuemin Pang, Yufeng He, Yuchen Wang, Tong Wang, Yong Li, Yunlong Si   +12 more
wiley   +1 more source

Most significantly enriched pathways analysis of cancer driver genes in shDMKN lentivirus (KD) and GFP lentivirus (NC) control in C8161 cell.

, 2023
Most significantly enriched pathways analysis of cancer driver genes in shDMKN lentivirus (KD) and GFP lentivirus (NC) control in C8161 cell.
Saber Imani (6680012), Qing Lian Wen (16528396), Qiao Weng (8370714), Junjiang Fu (8785076), Wenqiong Ma (13259891), Zexiu Wu (16528390), Mazaher Maghsoudloo (8322744), Iqra Ijaz (8040845), Marzieh Dehghan Shasaltaneh (16528393), Yuqin Zhang (522712)   +9 more
core   +1 more source

Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography

Veterinární Medicína, 2001
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal binding domain in pRSET-B vector.
D. Molinková
doaj   +1 more source