Results 41 to 50 of about 29,993 (267)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Assessment and Adoption of Cloud Computing among Academic Libraries in Philippines

University Library at a New Stage of Social Communications Development
Objective. Because cloud computing offers adaptable, scalable, and affordable technological solutions, it has profoundly changed academic librarianship.
E. D. MALABANAN
doaj   +1 more source

建立圖書館自動化系統的標準評鑑模式 Building a Standard Evaluative Model for Library Information System [PDF]

Jiàoyù zīliào yǔ túshūguǎn xué, 2002
無Library automation has been developed for 30 years nearly in Taiwan since 1973. after a serious literature review on the topic of library automation evaluation in Taiwan, only several papers have been discussed, but most for them focus on case study ...
Ya-ning Chen
doaj  

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Digital Tools of the Academic Library: Automation of Publication Activity Monitoring

University Library at a New Stage of Social Communications Development
Objective. This publication studies the potential for using artificial intelligence and open APIs (Application Programming Interface) to automate publication activity monitoring. Methods.
O. A. SHEVCHUK, A. A. SALTYKOVA
doaj   +1 more source

The introduction of library automation in the slovene National and University Library and other libraries (1977-1979)

Knjižnica, 2006
The article describes further efforts to implement library automation in the Slovene National and University Library and other libraries in the period from 1977 to 1979.
Stanislav Bahor
doaj   +1 more source

High-cytocompatible semi-IPN bio-ink with wide molecular weight distribution for extrusion 3D bioprinting

Scientific Reports, 2022
The development of 3D printing has recently attracted significant attention on constructing complex three-dimensional physiological microenvironments. However, it is very challenging to provide a bio-ink with cell-harmless and high mold accuracy during ...
Meiqi Li, Tingchun Shi, Danyu Yao, Xiuyan Yue, Haoxuan Wang, Kezhou Liu   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Risk Prediction Models for Recurrence After Curative Treatment of Early‐Stage or Locally Advanced Lung Cancer: A Systematic Review

Aging and Cancer, EarlyView.
This systematic review synthesizes prognostic models for survival and recurrence in resected non‐small cell lung cancer. While many models demonstrate moderate to good discrimination, few are externally validated and reporting quality is variable, limiting clinical applicability and highlighting the need for robust, transparent model development ...
Evangeline Samuel, S. Tissera, E. Paul, J. Zalcberg, R. G. Stirling   +4 more
wiley   +1 more source