Results 221 to 230 of about 1,177,346 (315)
Journal of Chemical Education, 2013 The experiment described here uses the binding of cyanine dyes to DNA as a model system for demonstrating the role that both ligand structure and noncovalent interactions play in DNA binding. Upper-division biochemistry laboratory students observe changes in absorbance and fluorescence spectra when small molecules bind either in the minor groove of DNA
Kristen C. Roy
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Journal of Chemical Education, 2005 Equilibrium gel filtration liquid chromatography is a technique used for the quantitation of ligand binding. We have designed an experiment for the senior undergraduate laboratory using this technique for the measurement of the binding of ATP to cytochrome c.
Douglas B. Craig
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Journal of Chemical Education, 2015 The interaction between oxygen and hemoglobin is a classic example of a cooperative ligand-binding process. This gaseous-ligand binding process may be studied in the undergraduate laboratory with a variable-pressure cuvette apparatus. An updated method is described to assemble a variable-pressure cuvette apparatus composed of inexpensive supplies and ...
Megan K. M. Young, Theodore J. Gries
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Progress in Lipid Research, 2006 2-Arachidonoylglycerol (2-AG) is a unique molecular species of monoacylglycerol isolated in 1995 from rat brain and canine gut as an endogenous ligand for the cannabinoid receptors. 2-AG is rapidly formed from arachidonic acid-containing phospholipids through increased phospholipid metabolism, such as enhanced inositol phospholipid turnover, in various
T. SUGIURA +3 more
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, 2006 The similarity in the functional spectra of many endogenous regulatory oligopeptides [1] allows the assumption that these molecules possess some common physicochemical properties. These properties provide the basis for the interaction of an oligopeptide with a particular set of receptor structures, and this interaction is the initial step of the ...
Andrey A. Zamyatnin, Olga L. Voronina
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Journal of Chemical Education, 2002 We have developed an experiment that utilizes the salt dependence of spermine binding to DNA as a means to introduce experimental design concepts and polyelectrolyte effects in the undergraduate biochemistry lab. The experiment is easily completed in two, three-hour lab periods.
Matthew A. Fisher +2 more
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Dinitrosyl iron complexes with thiolate ligands: Physico-chemistry, biochemistry and physiology
Nitric Oxide, 2009 Some present-day concepts on the origin and functional activities of dinitrosyl iron complexes (DNIC) with thiolate ligands are considered. Nitric oxide (NO) including to DNIC increases its stability and ensures effective targeting of NO to organs and tissues.
А. Ф. Ванин
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Histamine Receptors: Specific Ligands, Receptor Biochemistry, and Signal Transduction
, 2002 During the past few years, there has been a tremendous increase in our understanding of the histamine receptors. Important progress has been made in the development of H1-receptor agonists and the rationalization of H1-receptor-ligand interaction. The recent observation of constitutive H1- and H2-receptor activity has led to a reclassification of H1 ...
Remko A. Bakker, H. Timmerman, Rob Leurs
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Chemistry – A European Journal, 2011 AbstractThe presence of Fe‐bound cyanide ligands in the active site of the proton‐reducing enzymes [FeFe]‐hydrogenases has led to the hypothesis that such Brønsted–Lowry bases could be protonated during the catalytic cycle, thus implying that hydrogen isocyanide (HNC) might have a relevant role in such crucial microbial metabolic paths.
Claudio Greco +4 more
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