Results 261 to 270 of about 373,653 (310)

Equilibrium Gel Filtration Chromatography for the Measurement of Protein-Ligand Binding in the Undergraduate Biochemistry Laboratory

open access: closedJournal of Chemical Education, 2005
Equilibrium gel filtration liquid chromatography is a technique used for the quantitation of ligand binding. We have designed an experiment for the senior undergraduate laboratory using this technique for the measurement of the binding of ATP to cytochrome c.
Douglas B. Craig
openaire   +2 more sources

Histamine Receptors: Specific Ligands, Receptor Biochemistry, and Signal Transduction

open access: closed, 2002
During the past few years, there has been a tremendous increase in our understanding of the histamine receptors. Important progress has been made in the development of H1-receptor agonists and the rationalization of H1-receptor-ligand interaction. The recent observation of constitutive H1- and H2-receptor activity has led to a reclassification of H1 ...
Remko A. Bakker, H. Timmerman, Rob Leurs
openaire   +2 more sources

Isocyanide in Biochemistry? A Theoretical Investigation of the Electronic Effects and Energetics of Cyanide Ligand Protonation in [FeFe]‐Hydrogenases

open access: closedChemistry – A European Journal, 2011
AbstractThe presence of Fe‐bound cyanide ligands in the active site of the proton‐reducing enzymes [FeFe]‐hydrogenases has led to the hypothesis that such Brønsted–Lowry bases could be protonated during the catalytic cycle, thus implying that hydrogen isocyanide (HNC) might have a relevant role in such crucial microbial metabolic paths.
GRECO, CLAUDIO   +4 more
openaire   +3 more sources

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