Results 121 to 130 of about 153,107 (330)

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Protein neddylation in lung tumorigenesis: Target validation and targeted therapy

Fundamental Research
Much akin to ubiquitylation, neddylation is catalyzed by a cascade of three enzymes: E1 NEDD8-activating enzyme, E2 NEDD8-conjugating enzyme (UBE2M or UBE2F), and E3 NEDD8 ligases.
Yawen Zheng, Hiroyuki Inuzuka, Wenyi Wei, Yi Sun   +3 more
doaj   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

DNA ligases in the repair and replication of DNA

, 2000
DNA ligases are critical enzymes of DNA metabolism. The reaction they catalyse (the joining of nicked DNA) is required in DNA replication and in DNA repair pathways that require the re-synthesis of DNA.
David J Timson, Dale B Wigley, Timson, David J., Singleton, Martin R., Wigley, Dale B., Martin R Singleton   +5 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The ubiquitin symphony: E3 ligases as key conductors in the orchestra of growth and stress responses in fruits and vegetables

Horticultural Plant Journal
Fruits and vegetables undergo various regulatory mechanisms during growth, development, postharvest process and stress responses, all of which are crucial to their quality.
Durray Shahwar, Fujun Li, Xiaodong Fu, Yanan Li, Xinhua Zhang, Zienab Fawzy Reiad Ahmed   +5 more
doaj   +1 more source

Novel RING E3 Ubiquitin Ligases in Breast Cancer

Neoplasia: An International Journal for Oncology Research, 2006
Defects in ubiquitin E3 ligases are implicated in the pathogenesis of several human diseases, including cancer, because of their central role in the control of diverse signaling pathways.
Angelika Burger, Yutaka Amemiya, Richard Kitching, Arun K. Seth   +3 more
doaj   +1 more source

Ubiquitin ligases: guardians of mammalian development

Nature reviews. Molecular cell biology, 2022
David A. Cruz Walma, Zhuoyao Chen, A. Bullock, Kenneth M. Yamada   +3 more
semanticscholar   +1 more source

Ubiquitin ligases in cancer immunotherapy – balancing antitumor and autoimmunity

Trends in Molecular Medicine, 2019
Considerable progress has been made in understanding the contribution of E3 ubiquitin ligases to health and disease, including the pathogenesis of immunological disorders.
Y. Fujita, R. Tinoco, Yan Li, D. Senft, Z. Ronai   +4 more
semanticscholar   +1 more source

Role of E3 ligases on skeletal muscle plasticity.

, 2012
Neste estudo analisamos o envolvimento de E3 ligases sob três aspectos da plasticidade muscular esquelética: a perda de massa decorrente do desuso, a manutenção de fibras tipo I e II e a regeneração do tecido muscular.
Igor Luchini Baptista, Baptista, Igor Luchini   +1 more
core   +1 more source