Computational and Structural Biotechnology Journal, 2022 Protein ubiquitination plays a vital role in controlling the degradation of intracellular proteins and in regulating cell signaling pathways. Functionally, E3 ubiquitin ligases control the transfer of ubiquitin to the target substrates. As a major family
Xiaohua Lou +7 more
doaj
PUB-MS - a mass-spectrometry-based method to monitor protein-protein proximity in vivo [PDF]
arXiv, 2011 The common techniques to study protein-protein proximity in vivo are not well-adapted to the capabilities and the expertise of a standard proteomics laboratory, typically based on the use of mass spectrometry. With the aim of closing this gap, we have developed PUB-MS (for Proximity Utilizing Biotinylation and Mass Spectrometry), an approach to monitor
arxiv
Enzymatic breakage and joining of deoxyribonucleic acid. IV. DNA synthesis in E. coli infected with ligase-negative mutants of phage T4. [PDF]
, 1968 Yukito Masamune, C C Richardson
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TRIM56 coiled-coil domain structure provides insights into its E3 ligase functions
Computational and Structural Biotechnology Journal, 2023 Protein ubiquitination is a post-translation modification mediated by E3 ubiquitin ligases. The RING domain E3 ligases are the largest family of E3 ubiquitin ligases, they act as a scaffold, bringing the E2-ubiquitin complex and its substrate together to
Xiaohua Lou +7 more
doaj
Polynucleotide ligase from myeloid and lymphoid tissues. [PDF]
, 1968 Tomas Lindahl, Gerald M. Edelman
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A multi-lock inhibitory mechanism for fine-tuning enzyme activities of the HECT family E3 ligases
Nature Communications, 2019 HECT type E3 ligases are key regulators of cell growth and proliferation. Here the authors present the crystal structures of the Nedd4 family E3 ligase WWP1 in a closed and semi-open state and in combination with mutagenesis experiments identify a multi ...
Zhen Wang +9 more
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A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice
Cell Reports, 2019 Summary: Skeletal muscle cell (myofiber) atrophy is a detrimental component of aging and cancer that primarily results from muscle protein degradation via the proteasome and ubiquitin ligases.
Liam C. Hunt +11 more
doaj
Restoration by T4 ligase of DNA sequences sensitive to “flush”-cleaving restriction enzyme [PDF]
, 1977 Monica Mottes +3 more
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Bacteriophage T4 RNA ligase is gene 63 product, the protein that promotes tail fiber attachment to the baseplate. [PDF]
, 1977 Thomas J. Snopek +4 more
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Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase. [PDF]
, 1978 Yo Kikuchi +2 more
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