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The Staudinger Ligation

open access: yesChemical Reviews, 2020
While the Staudinger reaction has first been described a hundred years ago in 1919, the ligation reaction became one of the most important and efficient bioconjugation techniques in the 1990s and this century.
Nicole Jung, Stefan Bräse, Ute Schepers
exaly   +6 more sources

Comparison of Hi-C results using in-solution versus in-nucleus ligation. [PDF]

open access: yesGenome Biology, 2015
BACKGROUND: Chromosome conformation capture and various derivative methods such as 4C, 5C and Hi-C have emerged as standard tools to analyze the three-dimensional organization of the genome in the nucleus.
Fraser, Peter   +5 more
core   +3 more sources

Left lateral flank approach for spaying in cats [PDF]

open access: yesOpen Veterinary Journal, 2022
Background: Spaying is considered as a reliable surgical method for birth control and preventing potential feline reproductive diseases. Aim: This experiment was carried out to evaluate the suitability of left lateral flank approach for routine spaying
Mohammad Raguib Munif   +2 more
doaj   +1 more source

A Matched Case Control Study of Surgically and Non-surgically Treated Patent Ductus Arteriosus in Extremely Pre-term Infants

open access: yesFrontiers in Pediatrics, 2021
Introduction: There are still uncertainties about the timing and indication for surgical ligation of patent ductus arteriosus (PDA) in pre-term infants, where lower gestational age (GA) usually is predictive for surgical treatment.Objective: Our aim was ...
Karl Wilhelm Olsson   +4 more
doaj   +1 more source

A Ligation/Recombinase Polymerase Amplification Assay for Rapid Detection of SARS-CoV−2

open access: yesFrontiers in Cellular and Infection Microbiology, 2021
The pandemic of COVID-19 caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led to more than 117 million reported cases and 2.6 million deaths. Accurate diagnosis technologies are vital for controlling this pandemic.
Pei Wang   +8 more
doaj   +1 more source

Periodontitis induced by orthodontic wire ligature drives oral microflora dysbiosis and aggravates alveolar bone loss in an improved murine model

open access: yesFrontiers in Microbiology, 2022
AimTo assess the contribution of polymicrobial disruption of host homeostasis to periodontitis progression in orthodontic wire ligation murine model.MethodsOrthodontic wire rings were inserted between the first and second molars of mice for 18 days for ...
Rongshuang Ai   +7 more
doaj   +1 more source

Role of a Micronized Purified Flavonoid Fraction as an Adjuvant Treatment to Rubber Band Ligation for the Treatment of Patients With Hemorrhoidal Disease: A Longitudinal Cohort Study [PDF]

open access: yesAnnals of Coloproctology, 2019
Purpose Nonsurgical treatment of hemorrhoidal disease (HD) includes medical and instrumental techniques. We aimed to compare the efficacy of the most frequently used nonsurgical strategies, either alone or in combination, applied in an ambulatory setting.
Ana Célia Caetano   +4 more
doaj   +1 more source

Emergence of homochirality via template-directed ligation in an RNA reactor [PDF]

open access: yesPRX Life 2, 013015 (2024), 2023
RNA in extant biological systems is homochiral; it consists exclusively of D-ribonucleotides rather than L-ribonucleotides. How the homochirality of RNA emerged is not known. Here, we use stochastic simulations to quantitatively explore the conditions for RNA homochirality to emerge in the prebiotic scenario of an RNA reactor, in which RNA strands ...
arxiv   +1 more source

Ligation and Ligases [PDF]

open access: yesCold Spring Harbor Protocols, 2019
DNA ligases are used chiefly to create novel combinations of nucleic acid molecules and to attach them to vectors before molecular cloning. They are either of bacterial origin or bacteriophage encoded and have different properties, as discussed here.
Michael R. Green, Joseph Sambrook
openaire   +3 more sources

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