Results 1 to 10 of about 707,624 (278)
Chemical Reviews, 2020 While the Staudinger reaction has first been described a hundred years ago in 1919, the ligation reaction became one of the most important and efficient bioconjugation techniques in the 1990s and this century. It holds the crucial characteristics for bioorthogonal chemistry: biocompatibility, selectivity, and a rapid and high-yielding turnover for a ...
Nicole Jung, Stefan Bräse, Ute Schepers
exaly +6 more sources
Peptide ligation by chemoselective aminonitrile coupling in water
Nature, 2019 Matthew W Powner, Saidul Islam
exaly +2 more sources
Emergence of homochirality via template-directed ligation in an RNA reactor [PDF]
PRX Life 2, 013015 (2024), 2023 RNA in extant biological systems is homochiral; it consists exclusively of D-ribonucleotides rather than L-ribonucleotides. How the homochirality of RNA emerged is not known. Here, we use stochastic simulations to quantitatively explore the conditions for RNA homochirality to emerge in the prebiotic scenario of an RNA reactor, in which RNA strands ...
arxiv +1 more source
Cold Spring Harbor Protocols, 2019 DNA ligases are used chiefly to create novel combinations of nucleic acid molecules and to attach them to vectors before molecular cloning. They are either of bacterial origin or bacteriophage encoded and have different properties, as discussed here.
Michael R. Green, Joseph Sambrook
openaire +3 more sources
Temporal profile and mechanisms of the prompt sympathoexcitation following coronary ligation in Wistar rats [PDF]
, 2014 Our aim was to assess the timing and mechanisms of the sympathoexcitation that occurs immediately after coronary ligation. We recorded thoracic sympathetic (tSNA) and phrenic activities, heart rate (HR) and perfusion pressure in Wistar rats subjected to ...
Abdala, Ana Paula +5 more
core +10 more sources
Targeted nanopore sequencing with Cas9-guided adaptor ligation
Nature Biotechnology, 2020 Despite recent improvements in sequencing methods, there remains a need for assays that provide high sequencing depth and comprehensive variant detection.
Timothy Gilpatrick +9 more
semanticscholar +1 more source
On the Computational Power of DNA Annealing and Ligation [PDF]
, 1995 In [20] it was shown that the DNA primitives of Separate, Merge, and Amplify were not sufficiently powerful to invert functions defined by circuits in linear time.
Winfree, Erik
core +3 more sources
Tunable and Portable Extreme-Scale Drug Discovery Platform at Exascale: the LIGATE Approach [PDF]
, 2023 Today digital revolution is having a dramatic impact on the pharmaceutical industry and the entire healthcare system. The implementation of machine learning, extreme-scale computer simulations, and big data analytics in the drug design and development process offers an excellent opportunity to lower the risk of investment and reduce the time to the ...
arxiv +1 more source
Do KATP channels open as a prominent and early feature during ischaemia in the Langendorff-perfused rat heart? [PDF]
, 2000 The objective was to investigate whether myocardial adenosine triphosphate-sensitive K<sup>+</sup> (K<sub>ATP</sub>) channels open during the first 10 min of regional ischaemia in Langendorff-perfused rat hearts.
MacKenzie, I. +2 more
core +1 more source
Nucleic Acids Research, 2002 We describe a new method for relative quantification of 40 different DNA sequences in an easy to perform reaction requiring only 20 ng of human DNA. Applications shown of this multiplex ligation-dependent probe amplification (MLPA) technique include the ...
J. Schouten +5 more
semanticscholar +1 more source