Results 21 to 30 of about 357,308 (297)
BioTechniques, 2004 resultant fragments is further destroyed at its unique restriction-purification site. The crude digestion products are ligated in situ to generate a single plasmid containing the two fragments that can be selected for directly. Two of the two-fragment plasmids are then ligated to make a plasmid with a four-fragment insert, and the process is continued ...
Daniel V. Santi, Sarah J. Kodumal
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An Advance in Proline Ligation [PDF]
Journal of the American Chemical Society, 2011 Native chemical ligation (NCL) is widely applicable for building proteins in the laboratory. Since the discovery of this method, many strategies have been developed to enhance its capability and efficiency. Because of the poor reactivity of proline thioesters, ligation at a C-terminal proline site is not readily accomplished.
Samuel J. Danishefsky +4 more
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Advanced Biomedical Research, 2015 Background: Herniotomy is a common operation done by pediatric surgeons. Recent studies have shown that high ligation in herniation in adult is not necessary, but this method was not fully evaluated in children.
Mohammad Hadi Rafiei, Ali Jazini
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Management of Patent Ductus Arteriosus in Term or Near-term Neonates With Respiratory Distress
Pediatrics and Neonatology, 2010 Respiratory distress and patent ductus arteriosus (PDA) in neonates are mutually perpetuating. Contrary to the situation in premature infants, the recognition, clinical relevance and optimal management of PDA in full-term neonates are unclear.
Yu-Chen Lin +7 more
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Journal of Coloproctology, 2021 The doppler-guided transanal hemorrhoidal dearterialization technique associated with mucopexy is a noninvasive surgical option used to treat hemorrhoidal disease (HD).
Carlos Mateus Rotta +4 more
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The Latest Advance in Bracket Technology: Self-Ligating Brackets
Turkish Journal of Orthodontics, 2007 During the last 10 years, a wide range of metal, plastic, and ceramic brackets, based on the straight-wire system, have become available. The latest advance in bracket improvement is the development of self-ligating brackets in orthodontics.
Aylin Gökçelik, Ömür Polat
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Use of the Electrothermal Vessel Sealing System Versus Standard Vessel Ligation in Thyroidectomy
Asian Journal of Surgery, 2005 The electrothermal vessel sealing system (LigaSure(tm)) facilitates operative haemostasis by fusing blood vessel walls to form a collagen seal. The LigaSure(tm) is currently used in a variety of gastrointestinal, gynaecological and urological operations.
Wen T. Shen +3 more
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Laparoscopic repair of hernia in children: Comparison between ligation and nonligation of sac
Journal of Indian Association of Pediatric Surgeons, 2014 Aim: The essence of the current techniques of laparoscopic hernia repair in children is suture ligation of the neck of the hernia sac at the deep ring with or without its transection. Some studies show that during open hernia repair, after transection at
Nitin Pant +2 more
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Advances in Proline Ligation [PDF]
Journal of the American Chemical Society, 2012 Application of native chemical ligation logic to the case of an N-terminal proline is described. Two approaches were studied. One involved incorporation of a 3R-substituted thiyl-proline derivative. Improved results were obtained from a 3R-substituted selenol function, incorporated in the context of an oxidized dimer.
Suwei Dong +6 more
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