Results 31 to 40 of about 497,237 (348)

Endoscopic Management of Epistaxis in Lumbini Medical College

Journal of Lumbini Medical College, 2015
Introduction: Epistaxis is one of the commonest Ear Nose Throat (ENT) emergency. Proper guidelines for its management are lacking; on the other hand, the management is mostly done by the junior health service providers which has invited non-standardized ...
Anup Acharya, Madan Mohan Singh, Arati Shrestha   +2 more
doaj   +1 more source

Deep cervical lymph node ligation aggravates AD‐like pathology of APP/PS1 mice

Brain Pathology, 2018
The imbalance between production and clearance of amyloid‐beta (Aβ) is a key step in the onset and development of Alzheimer’s disease (AD). Therefore, reducing Aβ accumulation in the brain is a promising therapeutic strategy for AD.
Linmei Wang, Yanli Zhang, Ying Zhao, Charles Marshall, Ting-Yao Wu, Ming Xiao   +5 more
semanticscholar   +1 more source

Immobilization of Staphylococcus aureus Sortase A on Chitosan Particles and Its Applications in Peptide-to-Peptide Ligation and Peptide Cyclization

Molecules, 2018
Chitosan macro-particles prepared by the neutralization method were applied to Sortase A (SrtA) immobilization using glutaraldehyde as a crosslinking agent.
Min Yang, Haofei Hong, Shaozhong Liu, Xinrui Zhao, Zhimeng Wu   +4 more
doaj   +1 more source

Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly.

ACS Synthetic Biology, 2018
Synthetic biology relies on the manufacture of large and complex DNA constructs from libraries of genetic parts. Golden Gate and other Type IIS restriction enzyme-dependent DNA assembly methods enable rapid construction of genes and operons through one ...
V. Potapov, J. L. Ong, R. Kucera, B. Langhorst, Katharina Bilotti, J. Pryor, E. Cantor, B. Canton, Thomas F. Knight, T. C. Evans, G. Lohman   +10 more
semanticscholar   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Enzyme Treatment-Free and Ligation-Independent Cloning Using Caged Primers in Polymerase Chain Reactions

Molecules, 2011
A new simple scheme for constructing recombinant vectors that does not require any restriction enzyme, ligase, or any other special enzyme treatment has been developed.
Akinori Kuzuya, Keita Tanaka, Hitoshi Katada, Makoto Komiyama   +3 more
doaj   +1 more source

Effects of intestinal lymphatic ligation on intestinal immunity in rats with severe acute pancreatitis

FEBS Open Bio, 2021
Severe acute pancreatitis (SAP) is one of the most common diseases of the gastrointestinal tract, characterized by a complicated pathogenesis, multiple organ failure, and high mortality.
Yuanqi Liu, Li Chen, Lulu Wang, Yuxia Xiong   +3 more
doaj   +1 more source

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source

A Novel Cu²⁺ Quantitative Detection Nucleic Acid Biosensors Based on DNAzyme and “Blocker” Beacon

Foods, 2023
In this paper, a “turn-off” biosensor for detecting copper (II) ions based on Cu2+-dependent DNAzyme and a “blocker” beacon were developed. Upon the copper ion being added, the Cu2+-dependent DNAzyme substrate strand was irreversibly cleaved, thereby ...
Hanyue Zhang, Kai Dong, Shuna Xiang, Yingting Lin, Xiaoyan Cha, Ying Shang, Wentao Xu   +6 more
doaj   +1 more source

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate‐binding pocket

FEBS Letters, EarlyView.
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley   +1 more source