Results 31 to 40 of about 357,308 (297)

Endoscopic Management of Epistaxis in Lumbini Medical College

Journal of Lumbini Medical College, 2015
Introduction: Epistaxis is one of the commonest Ear Nose Throat (ENT) emergency. Proper guidelines for its management are lacking; on the other hand, the management is mostly done by the junior health service providers which has invited non-standardized ...
Anup Acharya, Madan Mohan Singh, Arati Shrestha   +2 more
doaj   +1 more source

Immobilization of Staphylococcus aureus Sortase A on Chitosan Particles and Its Applications in Peptide-to-Peptide Ligation and Peptide Cyclization

Molecules, 2018
Chitosan macro-particles prepared by the neutralization method were applied to Sortase A (SrtA) immobilization using glutaraldehyde as a crosslinking agent.
Min Yang, Haofei Hong, Shaozhong Liu, Xinrui Zhao, Zhimeng Wu   +4 more
doaj   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

Enzyme Treatment-Free and Ligation-Independent Cloning Using Caged Primers in Polymerase Chain Reactions

Molecules, 2011
A new simple scheme for constructing recombinant vectors that does not require any restriction enzyme, ligase, or any other special enzyme treatment has been developed.
Akinori Kuzuya, Keita Tanaka, Hitoshi Katada, Makoto Komiyama   +3 more
doaj   +1 more source

Effects of intestinal lymphatic ligation on intestinal immunity in rats with severe acute pancreatitis

FEBS Open Bio, 2021
Severe acute pancreatitis (SAP) is one of the most common diseases of the gastrointestinal tract, characterized by a complicated pathogenesis, multiple organ failure, and high mortality.
Yuanqi Liu, Li Chen, Lulu Wang, Yuxia Xiong   +3 more
doaj   +1 more source

A Bioorthogonal Quadricyclane Ligation

Journal of the American Chemical Society, 2011
New additions to the bioorthogonal chemistry compendium can advance biological research by enabling multiplexed analysis of biomolecules in complex systems. Here we introduce the quadricyclane ligation, a new bioorthogonal reaction between the highly strained hydrocarbon quadricyclane and Ni bis(dithiolene) reagents.
Carolyn R. Bertozzi, Ellen M. Sletten
openaire   +3 more sources

Mechanistic basis for inhibition of the extended‐spectrum β‐lactamase GES‐1 by enmetazobactam and tazobactam

FEBS Letters, EarlyView.
Antimicrobial resistance (AMR) is of huge importance, resulting in over 1 million deaths each year. Here, we describe how a new drug, enmetazobactam, designed to help fight resistant bacterial diseases, inhibits a key enzyme (GES‐1) responsible for AMR. Our data show it is a more potent inhibitor than the related tazobactam, with high‐level computation
Michael Beer, Philip Hinchliffe, Marko Hanževački, Christopher R. Bethel, Catherine L. Tooke, Marc W. Van der Kamp, Krisztina M. Papp‐Wallace, Robert A. Bonomo, Stuart Shapiro, Adrian J. Mulholland, James Spencer   +10 more
wiley   +1 more source

A Novel Cu²⁺ Quantitative Detection Nucleic Acid Biosensors Based on DNAzyme and “Blocker” Beacon

Foods, 2023
In this paper, a “turn-off” biosensor for detecting copper (II) ions based on Cu2+-dependent DNAzyme and a “blocker” beacon were developed. Upon the copper ion being added, the Cu2+-dependent DNAzyme substrate strand was irreversibly cleaved, thereby ...
Hanyue Zhang, Kai Dong, Shuna Xiang, Yingting Lin, Xiaoyan Cha, Ying Shang, Wentao Xu   +6 more
doaj   +1 more source

Cecal Ligation and Puncture [PDF]

Current Protocols in Immunology, 2010
AbstractThe cecum contains a high concentration of microbes, which are a combination of Gram‐negative and Gram‐positive flora. These bacteria range from anaerobic to facultative aerobic to aerobic organisms. In the procedure described in this unit, the ligation of the cecum produces a source of ischemic tissue as well as polymicrobial infection.
Matthew J. Delano, Philip A. Efron, Kindra M. Kelly-Scumpia, Lyle L. Moldawer, Alex G. Cuenca   +4 more
openaire   +3 more sources

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

FEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican, Yara Nabawi, William Hongyu Zhang, Dunja Petrovic, Markus Wehrmann, Sara Muñoz‐García, Seda Koyuncu, David Vilchez   +7 more
wiley   +1 more source