Results 171 to 180 of about 38,595 (231)

Generation of Self‐Organizing Macrovascular Constructs by Bioprinting Human iPSC‐Derived Mesodermal Progenitor Cells

Advanced Science, EarlyView.
ABSTRACT Vascularization remains a major obstacle in tissue engineering. Here, we introduce a bioprinting strategy to generate centimeter‐scale, self‐organizing “mother vessel” constructs from iPSC‐derived hiMPCs. By optimizing bioink composition, printing was accomplished in a single‐step approach. Within one week, hiMPCs differentiated into both CD31+
Leyla E. Dogan, Nathaly A. Chicaiza‐Cabezas, Florian Kleefeldt, Philipp Wörsdörfer, Jürgen Groll, Süleyman Ergün   +5 more
wiley   +1 more source

FOS3D: A Fluorescence‐Enabled Toolkit for Characterizing a Three‐dimensional Osteosarcoma Model

Advanced Science, EarlyView.
FOS3D describes fluorescent (F) osteosarcoma (OS) cells in a tri‐dimensional (3D) model. The study comprises three phases: development, where biofabrication parameters are tuned to achieve cytocompatibility and tumor‐specific mechanical properties in cell‐laden gelatin methacryloyl constructs; validation, where whole‐well fluorescence reading is ...
William Humble, Wiktor Zywicki, Enrico Lucarelli, Ania Naila Guerrieri, Francesca Taraballi, Gianluca Cidonio, Claudia Di Bella, Carmine Onofrillo, Andrea J. O'Connor, Serena Duchi   +9 more
wiley   +1 more source

3D evaluation of the extracellular matrix of hypoxic pancreatic islets using light sheet fluorescence microscopy. [PDF]

Islets
Ramirez M, Bastien E, Chae H, Gianello P, Gilon P, Bouzin C.   +5 more
europepmc   +1 more source

Intrinsically Mitochondria‐Targeting Nanozyme via Coordination‐Assembly of Natural Quercetin for Cascade Antioxidant Therapy of Cerebral Ischemia‐Reperfusion Injury

Advanced Science, EarlyView.
This study uncovers that quercetin naturally targets mitochondria. By coordinating quercetin with Fe3+, we engineer an ultrasmall cascade nanozyme (MCN) with superoxide dismutase‐catalase activities. MCN crosses the damaged blood–brain barrier, scavenges mitochondrial ROS, prevents mitochondrial DNA leakage, and blocks the cGAS‐STING pathway, thereby ...
Wenxuan Zheng, Zhicheng Wang, Xin Zhou, Shuya Wang, Xiaojing Shi, Tingli Xiong, Ruishi Li, Yuting Lin, Zhen Chen, Jiawen Wei, Fei Li, Jinwen Ge, Kelong Ai, Chong Liu, Guiming Deng   +14 more
wiley   +1 more source

An MR-based brain template and atlas for optical projection tomography and light sheet fluorescence microscopy in neuroscience. [PDF]

Front Neurosci
Willekens SMA, Morini F, Mediavilla T, Nilsson E, Orädd G, Hahn M, Chotiwan N, Visa M, Berggren PO, Ilegems E, Överby AK, Ahlgren U, Marcellino D.   +12 more
europepmc   +1 more source

Biodegradable Zn‐Based Implants: Progress, Challenges, and Pathways toward Clinical Translation

Advanced Science, EarlyView.
Exploring biodegradable Zn‐based implants offers a promising pathway to next‐generation biomedical devices with balanced degradation and biocompatibility. A comprehensive overview of biodegradable Zn‐based implants, covering their biological significance, material design principles, and advanced engineering strategies is provided.
Panfeng Zhao, Zhengquan Wang, Hui Yang, Xiyuan Zhang, Hanyuan Liu, Xiaotong Lu, Xiaocheng Li, Yanyan Liu, Xi Zhao, Wenhao Zhou, Yufeng Zheng   +10 more
wiley   +1 more source

TDP‐43 Aggregation: The Healthy‐Toxic Balance of the Prion‐Like Domain

Advanced Science, EarlyView.
TDP‐43 function relies on a delicate balance between reversible phase‐separated states and irreversible aggregation. Under physiological conditions, TDP‐43 forms dynamic droplets and oligomers that support normal cellular functions. In pathological contexts, this balance shifts toward aberrant aggregation, leading to toxic species.
Luca Zangrando, Emanuele Buratti, Francesca Paron   +2 more
wiley   +1 more source

A vascularized breast cancer spheroid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy. [PDF]

Nat Commun
Ascheid D, Baumann M, Pinnecker J, Friedrich M, Szi-Marton D, Medved C, Bundalo M, Ortmann V, Öztürk A, Nandigama R, Hemmen K, Ergün S, Zernecke A, Hirth M, Heinze KG, Henke E.   +15 more
europepmc   +1 more source

Conformational Transduction Amplification in a Biomimetic Polyelectrolyte for Ultrasensitive Imaging of Iron Metabolism

Advanced Science, EarlyView.
This work designs a peptide‐free polyelectrolyte with stable, switchable conformations and near‐infrared fluorescence in water. It harnesses a cooperative Fe3+‐triggered sheet‐to‐helix transition to generate nonlinear fluorescence quenching, enabling the ultrasensitive, real‐time visualization of Fe3+ ion distribution and metabolic trafficking at ...
Yeqiang Zhou, Danqi Yang, Yiwei Wang, Shuangyan Li, Fan Fan, Jiayu Zou, Cheng Zhang, Yang Liu, Hong Tan, Mingming Ding   +9 more
wiley   +1 more source

Pericentrosomal Redistribution of the Endoplasmic Reticulum Ensures Organelle Symmetric Inheritance and Mitotic Progression

Advanced Science, EarlyView.
Upon mitotic entry, RTN4 relocalizes to the pericentrosomal region, forming a more tubular ER network around centrosomes. CDK1‐mediated phosphorylation of RTN4 increases its interaction with Rab11 GTPase, facilitating dynein‐dependent transport of RTN4 to the pericentrosomal region.
Xiangyu Xu, Yalin Liu, Rongyi Wang, Wenwen Xu, Hao Shi, Ning Huang, Junlin Teng, Jin Meng, Pengli Zheng, Jianguo Chen   +9 more
wiley   +1 more source