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Practical considerations for quantitative light sheet fluorescence microscopy
Nature Methods, 2022Fluorescence microscopy has evolved from a purely observational tool to a platform for quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic imaging modalities has spurred a rapid growth in light sheet fluorescence microscopy (LSFM).
Chad M Hobson +2 more
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Light-sheet fluorescence microscopy for quantitative biology
Nature Methods, 2014In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.
Ernst H K Stelzer, Stelzer Ernst H K
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Comparison of optical projection tomography and light‐sheet fluorescence microscopy
Journal of Microscopy, 2019SummaryWe present a numerical analysis and experimental characterisation of spatial resolution in optical projection tomography (OPT) and light‐sheet fluorescence microscopy (LSFM) using their ‘standard’ systems. Although both techniques provide spatial resolution at the micrometre scale for mesoscopic (millimetre to centimetre) samples, LSFM provides ...
Aiping Liu, Renjian Li, Lingling Chen
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Doubling the resolution of light-sheet fluorescence microscopy
Nature Methods, 2022exaly +3 more sources
Fast Fluorescence Microscopy with Light Sheets
The Biological Bulletin, 2016In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. This illumination scheme provides minimal photobleaching and phototoxicity. Complemented with remote focusing and multi-view acquisition, light sheet microscopy is the method of choice for acquisition of
Daetwyler, S., Huisken, J.
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Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
Journal of Visualized Experiments, 2022Deafness is the most common sensory impairment, affecting approximately 5% or 430 million people worldwide as per the World Health Organization1. Aging or presbycusis is a primary cause of sensorineural hearing loss and is characterized by damage to hair cells, spiral ganglion neurons (SGNs), and the stria vascularis. These structures reside within the
Peter A, Santi, Shane B, Johnson
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Light-sheet fluorescence microscopy with metalens
ODS 2020: Industrial Optical Devices and Systems, 2020We propose a Light-sheet fluorescent microscopy (LSFM) system with a flat metalens, which is used to replace the bulky illuminating components. The metalens is a diffractive optics elements (DOEs) composed of various gallium nitride (GaN) nanopillar for light-sheet generation, and it can be readily integrated in existing LSFM systems.
Cheng Hung Chu +6 more
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Digital Scanned Laser Light Sheet Fluorescence Microscopy
Cold Spring Harbor Protocols, 2010INTRODUCTIONModern applications in the life sciences are frequently based on in vivo imaging of biological specimens, a domain for which light microscopy approaches are typically best suited. Often, quantitative information must be obtained from large multicellular organisms on the cellular or even subcellular level and with a good temporal resolution.
Philipp J, Keller, Ernst H K, Stelzer
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Light-sheet fluorescence microscopy for biomedicine
2023The research motivation behind this thesis is to expand the information that can currently be extracted from an ex vivo biological sample via light-sheet fluorescence microscopy (LSFM) by enhancing the achievable resolution at an increased penetration depth. LSFM has already been used considerably for various biomedical research applications.
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Light Sheet Fluorescence Microscopy of the Trabecular Meshwork in Rodent Eyes
Imaging and Applied Optics Congress, 2020Trabecular meshwork of the eye in Wistar rats was imaged using high-resolution, noncontact mode Bessel beam light sheet fluorescence microscopy. Distinct features of the trabecular meshwork can be observed using this technique.
Sandeep, C. S. Suchand +4 more
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