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Digital Scanned Laser Light Sheet Fluorescence Microscopy
Cold Spring Harbor Protocols, 2010INTRODUCTIONModern applications in the life sciences are frequently based on in vivo imaging of biological specimens, a domain for which light microscopy approaches are typically best suited. Often, quantitative information must be obtained from large multicellular organisms on the cellular or even subcellular level and with a good temporal resolution.
Philipp J, Keller, Ernst H K, Stelzer
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Light-sheet fluorescence microscopy for biomedicine
2023The research motivation behind this thesis is to expand the information that can currently be extracted from an ex vivo biological sample via light-sheet fluorescence microscopy (LSFM) by enhancing the achievable resolution at an increased penetration depth. LSFM has already been used considerably for various biomedical research applications.
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Light Sheet Fluorescence Microscopy of the Trabecular Meshwork in Rodent Eyes
Imaging and Applied Optics Congress, 2020Trabecular meshwork of the eye in Wistar rats was imaged using high-resolution, noncontact mode Bessel beam light sheet fluorescence microscopy. Distinct features of the trabecular meshwork can be observed using this technique.
Sandeep, C. S. Suchand +4 more
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Volume holographic optical element for light sheet fluorescence microscopy
Optics Letters, 2020Three-dimensional (3D) imaging of living organisms requires fine optical sectioning and high-speed image acquisition, which can be achieved by light sheet fluorescence microscopy (LSFM). However, orthogonal illumination and detection arms in the LSFM system make it bulky.
Ting-Yu, Hsieh +3 more
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Light sheet fluorescence microscopy (LSFM): past, present and future
The Analyst, 2014Light sheet fluorescence microscopy (LSFM) has emerged as an important imaging modality to follow biology in live 3D samples over time with reduced phototoxicity and photobleaching.
John, Lim +3 more
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Comparison of optical projection tomography and light‐sheet fluorescence microscopy
Journal of Microscopy, 2019SummaryWe present a numerical analysis and experimental characterisation of spatial resolution in optical projection tomography (OPT) and light‐sheet fluorescence microscopy (LSFM) using their ‘standard’ systems. Although both techniques provide spatial resolution at the micrometre scale for mesoscopic (millimetre to centimetre) samples, LSFM provides ...
A. LIU, W. XIAO, R. LI, L. LIU, L. CHEN
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Light sheet fluorescence microscopy with active optical manipulation
Optics LettersWe present a light sheet fluorescence microscopy (LSFM) with active optical manipulation by using linear optical tweezers (LOTs). In this method, two coaxially transmitting laser beams of different wavelengths are shaped using cylindrical lenses to form a linear optical trapping perpendicular to the optical axis and an excitation light sheet (LS ...
Jun, Yin +4 more
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Metalens-based light-sheet fluorescence microscopy
Optica Imaging Congress 2025 (3D, DH, COSI, IS, pcAOP,RadIT)Metalens-based dual-sided illumination light-sheet fluorescence microscopy utilizes paired cylindrical metalenses to generate uniform twin light sheets, eliminating shadow artifacts. The mouse lung tissues are measured by the system to offer high-resolution and sectioning imaging results.
Hung-Chuan Hsu +9 more
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Light-Sheet Fluorescence Microscopy
2022George O. T. Merces, Emmanuel G. Reynaud
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Light-Sheet Fluorescence Microscopy
Acta Optica Sinica, 2017杨豫龙 Yang Yulong +3 more
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